Whole Transcriptome RNA-Seq Analysis of Breast Cancer Recurrence Risk Using Formalin-Fixed Paraffin-Embedded Tumor Tissue

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Summary

This work has developed and optimized RNA-Seq library chemistry as well as bioinformatics and biostatistical methods for whole transcriptome profiling from FFPE tissue, which enable rediscovery of RNA biomarkers for disease recurrence risk that were previously identified by RT-PCR analysis of a cohort of 136 patients and identifies a high percentage of recurrencerisk markers previously discovered using DNA microarrays.

Type
article
Published
2012-07-13
Cited by
78
References
55
Access
Open access

Keywords

Foothills, Population, Geography, Stage (stratigraphy), Physical geography

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