Asymmetric siRNA: New Strategy to Improve Specificity and Reduce Off-Target Gene Expression
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Summary
This work demonstrated that structurally asymmetric siRNA targeting CCR5 could be adopted to improve the strand specificity and greatly reduce the off-target effects without significantly compromising its on-target effects.
- Type
- article
- Published
- 2012-01-09
- Cited by
- 24
- References
- 39
- Access
- Open access
- OpenAlex
- https://openalex.org/W22229698
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:19858111
Keywords
Accounting, Business
References
- Complete knockdown of CCR5 by lentiviral vector-expressed siRNAs and protection of transgenic macrophages against HIV-1 infection
- Measuring HIV neutralization in a luciferase reporter gene assay.
- siRNA-DIRECTED INHIBITION OF HIV-1 INFECTION
- Strategies for silencing human disease using RNA interference
- A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells
- Mechanisms of gene silencing by double-stranded RNA
- Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain
- A Protein Sensor for siRNA Asymmetry
- Molecular basis for target RNA recognition and cleavage by human RISC.
- Synthetic shRNAs as potent RNAi triggers
- Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.
- Structural determinants of RNA recognition and cleavage by Dicer
- Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA
- The promises and pitfalls of RNA-interference-based therapeutics
- Asymmetric shorter-duplex siRNA structures trigger efficient gene silencing with reduced nonspecific effects.
- Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy
- Small RNA sorting: matchmaking for Argonautes
- Effect of asymmetric terminal structures of short RNA duplexes on the RNA interference activity and strand selection
- RNA interference remarkably suppresses bcl-2 gene expression in cancer cells in vitro and in vivo
- Asymmetric RNA duplexes mediate RNA interference in mammalian cells
Cited by
- Computational methods for integrating and analyzing human systems biology data
- Type 2 diabetes mellitus: limitations of conventional therapies and intervention with nucleic acid-based therapeutics.
- Knockdown of glucose-regulated protein 78/binding immunoglobulin heavy chain protein expression by asymmetric small interfering RNA induces apoptosis in prostate cancer cells and attenuates migratory capability.
- MicroRNAs and multiple sclerosis: from physiopathology toward therapy
- Reconsideration of in silico siRNA design from a perspective of heterogeneous data integration: problems and solutions
- Strategic Applications of Gene Expression: From Drug Discovery/Development to Bedside
- siRNA and RNAi optimization
- Evaluation of inhibitory effect of shRNA constructs in bluetongue virus (BTV) Replication
- Synthetic SiRNA Delivery: Progress and Prospects.
- Circular siRNAs for Reducing Off-Target Effects and Enhancing Long-Term Gene Silencing in Cells and Mice
- Combined shRNA over CRISPR/cas9 as a methodology to detect off-target effects and a potential compensatory mechanism
- Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors.
- Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents.
- Small interfering RNAs (siRNAs) based gene silencing strategies for the treatment of glaucoma: Recent advancements and future perspectives.
- Chemical strategies for strand selection in short-interfering RNAs
- Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases
- Specificity of oligonucleotide gene therapy (OGT) agents
- Engineering a Novel Self-Assembled Multi-siRNA Nanocaged Architecture with Controlled Enzyme-Mediated siRNA Release
- Chemistry, structure and function of approved oligonucleotide therapeutics
- Using Transcriptome Diversity to Evaluate the Off-Target Effects of miRNA-Mediated Transgenic Rice.
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