Protein purification by dye-ligand chromatography.
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Summary
Dye-ligand chromatography has been demonstrated to be suitable for large-scale protein purification due to their high selectivity, stability, and economy and the issue of dye leakage and process validation of large- scale dye-ligands chromatography have been discussed.
- Type
- review
- Published
- 1993-01-01
- Cited by
- 39
- References
- 172
- OpenAlex
- https://openalex.org/W21120725
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:34046969
Keywords
Chemistry, Affinity chromatography, Ligand (biochemistry), Elution, Chromatography
References
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- Adsorption equilibrium in immuno‐affinity chromatography with polyclonal and monoclonal antibodies
- New strategies for the screening of a large number of immobilized dyes for the purification of enzymes. Application to the purification of enzymes from human haemolysate.
- The selective retardation of NADP+-dependent dehydrogenases by immobilized procion red HE-3B.
- Affinity chromatography of kinases and dehydrogenases on Sephadex and Sepharose dye derivatives.
- Theory and practice in affinity techniques
- Reactive Dyes in Protein and Enzyme Technology
- High-performance liquid chromatography of amino acids, peptides and proteins. XCII Thermodynamic and kinetic investigations on rigid and soft affinity gels with varying particle and pore sizes.
- Studies on the nature of transition-metal-ion-mediated binding of triazine dyes to enzymes. The interaction of procion red MX-8B with carboxypeptidase G-2.
- Design and applications of biomimetic anthraquinone dyes : II. The interaction of C.I. reactive blue 2 analogues bearing terminal ring modifications with horse liver alcohol dehydrogenase
- Dinucleotide fold proteins. Interaction of arabinose binding protein with Cibacron Blue 3G-A.
- Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation. Simultaneous purification of lactate dehydrogenase.
- Design and applications of biomimetic anthraquinone dyes : I. Synthesis and characterisation of terminal ring isomers of C.I. Reactive Blue 2
- Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes.
- Design and applications of biomimetic anthraquinone dyes. III. Anthraquinone-immobilised C.I. reactive blue 2 analogues and their interaction with horse liver alcohol dehydrogenase and other adenine nucleotide-binding proteins.
- Accessibility and multivalency of immobilized Cibacron blue F3GA.
Cited by
- Progress in monitoring, modeling and control of bioprocesses during the last 20 years.
- Process integration of cell disruption and fluidised bed adsorption of microbial enzymes: application to the retro-design of the purification of L-asparaginase
- Adsorptive recovery of nanoparticulate protein products : physical and biochemical characterisation of candidate solid phases
- Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand.
- Integration of mechanical cell disruption and fluidised bed recovery of G3PDH from unclarified disrupted yeast: a comparative study of the performance of unshielded and polymer shielded dye-ligand chromatography systems.
- Recent Developments in the Use of Group-Specific Ligands for Affinity Bioseparations
- Mass spectrometric noncovalent probing of amino acids in peptides and proteins
- Direct recovery of alcohol dehydrogenase from unclarified yeast cell homogenate by IDEBAC using an improved scheme for elution
- Binding of 5-(2'-carboxyphenyl)azoquinolin-8-ol to bovine serum albumin: a spectroscopic study.
- Protein structure information from mass spectrometry? Selective titration of arginine residues by sulfonates
- Purification and characterisation of isocitrate dehydrogenase and malate dehydrogenase from Mycobacterium tuberculosis and evaluation of their potential as suitable antigens for the serodiagnosis of tuberculosis.
- Albumin separation with Cibacron Blue carrying macroporous chitosan and chitin affinity membranes
- Boronate-Containing Copolymer Grafted on Eupergit C as Matrix for Affinity Chromatography: Isotherms and Kinetics Study
- Purification of neutral protease by dye affinity chromatography
- Dye-ligand affinity systems.
- Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
- FUNGAL PECTIC ENZYME FRACTIONATION BY DYE AFFINITY CHROMATOGRAPHY
- Expanded bed affinity chromatography of dehydrogenases from bakers' yeast using dye-ligand perfluoropolymer supports.
- Triazinic dye ligand selection by surface plasmon resonance for recombinant lactoferricin purification
- Enzymatic Regeneration of NAD in Enantioselective Oxidation of Secondary Alcohols: Glutamate Dehydrogenase Versus NADH Dehydrogenase
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