Retroviral transduction of hematopoietic progenitors derived from human embryonic stem cells.
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Summary
Detailed methods on the recently characterized and optimized GALV-pseudotyped retroviral gene transfer strategy to stably transduce the hematopoietic progenitor cells prospectively derived from CD45(neg)PFV hemogenic precursors are described.
- Type
- article
- Published
- 2006-01-01
- Cited by
- 1
- References
- 38
- Access
- Open access
- OpenAlex
- https://openalex.org/W16881519
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7187060
Keywords
The Internet, Computer science, World Wide Web, Information retrieval, Multimedia
References
- Human embryonic stem cells: culture, differentiation, and genetic modification for regenerative medicine applications.
- Sonic hedgehog induces the proliferation of primitive human hematopoietic cells via BMP regulation
- High‐Level Sustained Transgene Expression in Human Embryonic Stem Cells Using Lentiviral Vectors
- Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus
- ES cells have only a limited lymphopoietic potential after adoptive transfer into mouse recipients.
- Clonal analysis of differentiating embryonic stem cells reveals a hematopoietic progenitor with primitive erythroid and adult lymphoid-myeloid potential.
- Hematopoiesis from embryonic stem cells: lessons from and for ontogeny.
- Human homologues of Delta-1 and Delta-4 function as mitogenic regulators of primitive human hematopoietic cells.
- HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors.
- Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells.
- Stable genetic modification of human embryonic stem cells by lentiviral vectors.
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
- Homologous recombination in human embryonic stem cells
- Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells
- Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes.
- Peripheral blood stem cell versus bone marrow allotransplantation: does the source of hematopoietic stem cells matter?
- Side effects of retroviral gene transfer into hematopoietic stem cells.
- The Notch Ligand Jagged-1 Represents a Novel Growth Factor of Human Hematopoietic Stem Cells
- Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties.
- Introduction: spatial origin of murine hematopoietic stem cells.
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