The Lec4A CHO glycosylation mutant arises from miscompartmentalization of a Golgi glycosyltransferase
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Summary
The identification of the Lec4A defect indicates that appropriate screening of different glycosylation-defective mutants should enable the isolation of other mammalian cell trafficking mutants.
- Type
- article
- Published
- 1989-11-01
- Cited by
- 50
- References
- 31
- Access
- Open access
- OpenAlex
- https://openalex.org/W2530238
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:478408
Keywords
Representation (politics), Political science, Prime (order theory), Political economy, Economics
References
- Degradation from the endoplasmic reticulum: disposing of newly synthesized proteins.
- Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
- Evidence for extensive subcellular organization of asparagine-linked oligosaccharide processing and lysosomal enzyme phosphorylation.
- Selection of lectin-resistant mutants of animal cells.
- Lec1A Chinese hamster ovary cell mutants appear to arise from a structural alteration in N-acetylglucosaminyltransferase I.
- Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein.
- Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes.
- Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins.
- A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.
- Mutant of Chinese hamster ovary cells with altered mannose 6-phosphate receptor activity is unable to synthesize mannosylphosphoryldolichol.
- 1H NMR spectroscopy of carbohydrates from the G glycoprotein of vesicular stomatitis virus grown in parental and Lec4 Chinese hamster ovary cells.
- Carbohydrate heterogeneity of vesicular stomatitis virus G glycoprotein allows localization of the defect in a glycosylation mutant of CHO cells.
- Microheterogeneity among carbohydrate structures at the cell surface may be important in recognition phenomena.
- Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
- Mitogenic leukoagglutinin from Phaseolus vulgaris binds to a pentasaccharide unit in N-acetyllactosamine-type glycoprotein glycans.
- In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.
- Characterization of protein transport between successive compartments of the Golgi apparatus: asymmetric properties of donor and acceptor activities in a cell-free system.
- Complementation between mutants of CHO cells resistant to a variety of plant lectins
- Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cell.
Cited by
- Localisation of the multidrug resistance-associated protein, MRP, in resistant large-cell lung tumour cells.
- Mechanism of the Facilitation of PC2 Maturation by 7B2: Involvement in ProPC2 Transport and Activation but Not Folding
- The regulation of cell- and tissue-specific expression of glycans by glycosyltransferases.
- Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase.
- Rapid Assays for Lectin Toxicity and Binding Changes that Reflect Altered Glycosylation in Mammalian Cells
- A single tryptophan residue of endomannosidase is crucial for Golgi localization and in vivo activity
- Deletion of Mouse Embryo Fibroblast N-Acetylglucosaminyltransferase V Stimulates α5β1 Integrin Expression Mediated by the Protein Kinase C Signaling Pathway*
- Glycosylation of recombinant proteins: problems and prospects.
- N-Glycans in cancer progression.
- Tumor cell surface β1–6 branched oligosaccharides and lung metastasis
- Regulation of Homotypic Cell-Cell Adhesion by Branched N-Glycosylation of N-cadherin Extracellular EC2 and EC3 Domains*
- Generation of antibodies recognizing an aberrant glycoform of human tissue inhibitor of metalloproteinase-1 (TIMP-1) using decoy immunization and phage display.
- Preparation of antisera to recombinant, solubleN-acetylglucosaminyltransferase V and its visualizationin situ
- A Point Mutation Causes Mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese Hamster Ovary Glycosylation Mutant*
- Increased fucosylation of high‐molecular‐weight glycoproteins accompanies retinoic‐acid‐induced differentiation of f‐9 embryonal carcinoma cells
- The Enzymatic Basis for the Conversion of Nonfucosylated to Fucosylated α-Fetoprotein by Acyclic Retinoid Treatment in Human Hepatoma Cells: Activation of α1-6 Fucosyltransferase
- Removal of sialic acid involving Klotho causes cell-surface retention of TRPV5 channel via binding to galectin-1
- Lectin-resistant CHO cells: Selection of seven new mutants resistant to ricin
- Characterization of Herpes Simplex Virus-Containing Organelles by Subcellular Fractionation: Role for Organelle Acidification in Assembly of Infectious Particles
- Reduction in Golgi apparatus dimension in the absence of a residential protein, N-acetylglucosaminyltransferase V