HIV-1 drug resistance genotyping success rates and correlates of Dried-blood spots and plasma specimen genotyping failure in a resource-limited setting
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Summary
Improve in DBS handling, pre-genotyping viral load testing to screen samples to enhance genotyping success and the development of more sensitive assays with well-designed primers to genotype specimens with low or undetectable viral load are recommended.
- Type
- article
- Published
- 2022-02-16
- Cited by
- 13
- References
- 49
- Access
- Open access
- OpenAlex
- https://openalex.org/W4280553679
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:246932617
Keywords
Genotyping, Viral load, Dried blood spot, Medicine, Drug resistance
References
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- HIV-1 drug resistance surveillance using dried whole blood spots
- Update on HIV-1 acquired and transmitted drug resistance in Africa.
- The use of dried blood spot specimens for HIV-1 drug resistance genotyping in young children initiating antiretroviral therapy
- Dried blood spots for HIV‐1 drug resistance genotyping in decentralized settings in Senegal
- Uganda's New National Laboratory Sample Transport System: A Successful Model for Improving Access to Diagnostic Services for Early Infant HIV Diagnosis and Other Programs
- Pyrosequencing Dried Blood Spots Reveals Differences in HIV Drug Resistance between Treatment Naïve and Experienced Patients
- Evaluation of a dried blood spot HIV-1 RNA program for early infant diagnosis and viral load monitoring at rural and remote health care facilities
- Comparison of HIV-1 RNA level estimated with plasma and DBS samples: a pilot study from India (South).
- Evaluation of dried blood spot specimens for HIV-1 drug-resistance testing using the Trugene HIV-1 genotyping assay.
- Dried blood spots versus plasma for the quantitation of HIV-1 RNA using a real-Time PCR, m2000rt assay.
- Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
- Plasma viraemia as a marker of viral replication in HIV-infected individuals.
- Automating HIV Drug Resistance Genotyping with RECall, a Freely Accessible Sequence Analysis Tool
- Dried blood spot in the genotyping, quantification and storage of HCV RNA: a systematic literature review
- High concordance between HIV-1 drug resistance genotypes generated from plasma and dried blood spots in antiretroviral-experienced patients
- Use of Dried-Blood-Spot Samples and In-House Assays To Identify Antiretroviral Drug Resistance in HIV-Infected Children in Resource-Constrained Settings
- HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4°C
- Field Study of Dried Blood Spot Specimens for HIV-1 Drug Resistance Genotyping
- HIV-1 drug resistance testing from dried blood spots collected in rural Tanzania using the ViroSeq HIV-1 Genotyping System
Cited by
- The plasma separation card as a novel solution for enhancing central laboratory capability for HIV-1 viral load monitoring in limited-access settings
- Assessment of the performance of the plasma separation card for HIV-1 viral load monitoring in South Africa
- A Validated In-House Assay for HIV Drug Resistance Mutation Surveillance from Dried Blood Spot Specimens.
- HIV-1 pretreatment and acquired antiretroviral drug resistance before tenofovir/ lamivudine /dolutegravir (TLD) roll-out in Mozambique
- The utility of integrating nanopore sequencing into routine HIV-1 drug resistance surveillance
- Barriers to effective sample management in viral load testing in Machakos County, Kenya: a convergent parallel mixed-method study
- HIV-1 drug resistance among people living with HIV receiving dolutegravir-based anti-retroviral regimens in Uganda: a national laboratory-based survey using remnant viral load samples, 2022
- HIV-1 genetic diversity and pretreatment drug resistance survey prior to dolutegravir introduction in Senegal
- Comparison of Plasma, Dried Blood Spots, and Peripheral Blood Mononuclear Cells as Biosamples for HIV-1 Genotypic Drug Resistance in a Tertiary Care Center
- VANTAGE: Van-Based Real-Time HIV Sequencing for Transmission Mapping and Drug Resistance Profiling in War-Affected Ukraine
- Evaluation of two bioinformatic algorithms for the interpretation of HIV-1 drug resistance and subtyping in Cameroon: Translational application for ART optimization in low-and middle-income countries.
- Emerging HIV-1 Drug Resistance during Persistent Low-Level Viremia is Associated with Elevated Viral loads among Individuals on First-line Antiretroviral Therapy in Uganda
- Additional file 1 of HIV-1 pretreatment and acquired antiretroviral drug resistance before tenofovir/ /lamivudine /dolutegravir (TLD) roll-out in Mozambique
- Additional file 1 of HIV-1 pretreatment and acquired antiretroviral drug resistance before tenofovir/ lamivudine /dolutegravir (TLD) roll-out in Mozambique
- HIV-1 Pretreatment and Acquired Antiretroviral Drug Resistance before tenofovir/ /lamivudine /dolutegravir (TLD) roll-out in Mozambique Running title: Pretreatment and Acquired HIV Drug Resistance in Mozambique
- Genotype-Guided Antiretroviral Therapy after Virologic Failure: A Retrospective Study of Virological Suppression at 6 and 12 Months in Uganda
- Additional file 1 of HIV-1 pretreatment and acquired antiretroviral drug resistance before tenofovir/ lamivudine /dolutegravir (TLD) roll-out in Mozambique
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