Fluorescence complementation enables quantitative imaging of cell penetrating peptide-mediated protein delivery in plants including WUSCHEL transcription factor
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Summary
A GFP bimolecular fluorescence complementation-based tool, delivered complementation in planta (DCIP), which allows for unambiguous and quantitative measurement of protein delivery in leaves and offers a new and powerful tool for interrogating cytosolic delivery of proteins in plants.
- Type
- preprint
- Published
- 2023-04-21
- Cited by
- 0
- References
- 70
- Access
- Open access
- OpenAlex
- https://openalex.org/W4229031701
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:258313510
Keywords
Bimolecular fluorescence complementation, Nicotiana benthamiana, mCherry, Cell-penetrating peptide, Gene delivery
References
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- Clathrin-independent endocytosis contributes to uptake of glucose into BY-2 protoplasts.
- Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation.
- Lifeact: a versatile marker to visualize F-actin
- WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators
- Mesoporous Silica Nanoparticle-Mediated Intracellular Cre Protein Delivery for Maize Genome Editing via loxP Site Excision1,2[W][OPEN]
- Fluorescent fusion protein knockout mediated by anti-GFP nanobody
- Uptake of fluorescent nano beads into BY2‐cells involves clathrin‐dependent and clathrin‐independent endocytosis
- GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology1[C][W][OA]
- Peptide degradation is a critical determinant for cell-penetrating peptide uptake.
- Noncovalent protein transduction in plant cells by macropinocytosis.
- Use of Ranks in One-Criterion Variance Analysis
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
- Targeting of TMV Movement Protein to Plasmodesmata Requires the Actin/ER Network; Evidence From FRAP
- Stability of cell-penetrating peptide-morpholino oligomer conjugates in human serum and in cells.
- Translocation and nuclear accumulation of monomer and dimer of HIV-1 Tat basic domain in triticale mesophyll protoplasts.
- Ligation-independent cloning of PCR products (LIC-PCR).
- The uptake of arginine-rich cell-penetrating peptides: putting the puzzle together.
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