Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing
Explore this paper's citation graph
Summary
The design, construction, and validation of a new dicistronic mCherry-lacZα reporter system by artificial lac operon and pbr operon models in lacZM15-producing E. coli offers a robust tool for biological studies.
- Type
- article
- Published
- 2020-01-30
- Cited by
- 22
- References
- 34
- Access
- Open access
- OpenAlex
- https://openalex.org/W3003335489
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:210984365
Keywords
mCherry, lac operon, Reporter gene, Operon, Biology
References
- Experiments in molecular genetics
- Quantitative, Non-Disruptive Monitoring of Transcription in Single Cells with a Broad-Host Range GFP-luxCDABE Dual Reporter System
- Tuning gene expression with synthetic upstream open reading frames
- Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression
- Monitoring of Gene Expression in Bacteria during Infections Using an Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion Vectors
- Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells
- Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression
- Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
- Tuning constitutive recombinant gene expression in Lactobacillus plantarum
- High-throughput single-cell analysis of low copy number β-galactosidase by a laboratory-built high-sensitivity flow cytometer.
- A GFP-lacZ Bicistronic Reporter System for Promoter Analysis in Environmental Gram-Negative Bacteria
- Simple whole-cell biodetection and bioremediation of heavy metals based on an engineered lead-specific operon.
- Detection of low-level promoter activity within open reading frame sequences of Escherichia coli
- Single-Cell Microbiology: Tools, Technologies, and Applications
- Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli
- agr Expression Precedes Escape of InternalizedStaphylococcus aureus from the Host Endosome
- Microbial reporters of metal bioavailability
- Molecular Cloning: A Laboratory Manual
- Control of MYEOV Protein Synthesis by Upstream Open Reading Frames*
- Translation of ‘rare’ Codons in a Cell-free Protein Synthesis System from Escherichia coli
Cited by
- Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor
- Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Cad Operons
- In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook
- Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury
- Development of a bioavailable Hg(II) sensing system based on MerR-regulated visual pigment biosynthesis
- Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor
- Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review
- A tailored Indigoidine-based whole-cell biosensor for detecting toxic cadmium in environmental water samples
- Anthocyanin biosynthetic pathway switched by metalloregulator PbrR to enable a biosensor for the detection of lead toxicity
- Designed bacteria based on natural pbr operons for detecting and detoxifying environmental lead: A mini-review.
- Tailored bacteria tackling with environmental mercury: Inspired by natural mercuric detoxification operons.
- Synthetic bacteria designed using ars operons: a promising solution for arsenic biosensing and bioremediation
- A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus neoformans
- Metabolic engineering-enabled dual-color biosensor for discriminative and sensitive detection of toxic lead and mercury in environmental waters
- PlomBOX: a low cost bioassay for the sensitive detection of lead in drinking water
- Development of a user-friendly IdgS-Sfp based whole-cell biosensor for high-performance Hg(II) detection in environmental samples
- Systematic evaluation of pigment-based whole-cell lead biosensors: challenges in genetic circuit engineering and critical considerations for background noise control
- Yeast Biosensors for the Safety of Fermented Beverages
- In-Situ Monitoring of Xenobiotics using Genetically Engineered Whole-Cell-Based Microbial Biosensors: Recent Advances and Outlook
Related papers
- Oxygen-regulated stimulons of Salmonella typhimurium identified by Mu d(Ap lac) operon fusions
- Regulation of the Ubiquinone (Coenzyme Q) Biosynthetic Genes ubiCA in Escherichiacoli
- Selection of lacZ operon fusions in genes of gluconate metabolism in E. coli. characterization of a gntT::lacZ fusion.
- Mud(Ap, lac)-generated fusions in studies of gene expression.
- The promoter-operator region of the lac operon of Escherichia coli.
- Prospect of the Lac Operon Mediated Galactosidase Applied in Transgenic Animals
- Bacillus subtilis pur operon expression and regulation
- Improved Monitoring of Low-Level Transcription in Escherichia coli by a β-Galactosidase α-Complementation System
- Angestaubt, aber nicht eingerostet — der Bioreporter LacZ