A simple bead-based method for generating cost-effective co-barcoded sequence reads
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Summary
Single tube long fragment read (stLFR), a simple barcoded bead-based process capable of near perfect whole genome variant calling and haplotyping and expects in the future that this process will enable affordable diploid de novo assembly.
- Type
- article
- Published
- 2018-10-11
- Cited by
- 6
- References
- 31
- Access
- Open access
- OpenAlex
- https://openalex.org/W2896058170
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:57452479
Keywords
Bead, Simple (philosophy), Sequence (biology), Computer science, Computational biology
References
- Haplotyping germline and cancer genomes using high-throughput linked-read sequencing
- Direct determination of molecular haplotypes by chromosome microdissection
- Co-barcoded sequence reads from long DNA fragments: a cost-effective solution for “perfect genome” sequencing
- Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing
- Long-range polony haplotyping of individual human chromosome molecules
- Truncating mutations of MAGEL2cause Prader-Willi phenotypes and autism
- Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
- Haplotype-resolved whole genome sequencing by contiguity preserving transposition and combinatorial indexing
- Haplotype-resolved genome sequencing of a Gujarati Indian individual
- Whole-genome molecular haplotyping of single cells
- Accurate whole genome sequencing and haplotyping from10-20 human cells
- A comprehensively molecular haplotype-resolved genome of a European individual.
- The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.
- Fosmid-based whole genome haplotyping of a HapMap trio child: evaluation of Single Individual Haplotyping techniques
- Detection and phasing of single base de novo mutations in biopsies from human in vitro fertilized embryos by advanced whole-genome sequencing
- Whole genome sequence analysis of BT-474 using complete Genomics’ standard and long fragment read technologies
- Premalignant SOX2 overexpression in the fallopian tubes of ovarian cancer patients: Discovery and validation studies
- The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes
- HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies
- Long Fragment Read (LFR) Technology: Cost-Effective, High-Quality Genome-Wide Molecular Haplotyping.
Cited by
- Efficient long single molecule sequencing for cost effective and accurate sequencing, haplotyping, and de novo assembly
- Efficient and unique cobarcoding of second-generation sequencing reads from long DNA molecules enabling cost-effective and accurate sequencing, haplotyping, and de novo assembly
- Chromosome level draft genomes of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), an alien invasive pest in China
- High-resolution single-molecule long-fragment rRNA gene amplicon sequencing for uncultured bacterial and fungal communities
- High-resolution single-molecule long-fragment rRNA gene amplicon sequencing of bacterial and eukaryotic microbial communities.
- Assembly and analysis of the genome of Notholithocarpus densiflorus
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