Developing synthetic hybrid promoters to increase constitutive or diauxic shift-induced expression in Saccharomyces cerevisiae.
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Summary
This study sought to overcome the limitations of native promoters in Saccharomyces cerevisiae through the construction of hybrid promoter libraries for both constitutive promoters and promoters induced by diauxic shift with a broad range of transcriptional capacity.
- Type
- article
- Published
- 2018-09-10
- Cited by
- 21
- References
- 30
- Access
- Open access
- OpenAlex
- https://openalex.org/W2891858816
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:52182678
Keywords
Promoter, Biology, Saccharomyces cerevisiae, Gene expression, Gene
References
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- Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA box".
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- Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistance.
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- Alcohol and lipid metabolism
- Vectors for Glucose-Dependent Protein Expression in Saccharomyces cerevisiae
- Global gene expression during short‐term ethanol stress in Saccharomyces cerevisiae
- Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae
- Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae.
- GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences.
- Shuffling of Promoters for Multiple Genes To Optimize Xylose Fermentation in an Engineered Saccharomyces cerevisiae Strain
- Carbon source dependent promoters in yeasts
- Tuning Gene Expression in Yarrowia lipolytica by a Hybrid Promoter Approach
- Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of l-Arabinose
- Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase
Cited by
- Engineered reversal of function in glycolytic yeast promoters
- Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues
- A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae
- Programmable Synthetic Upstream Activating Sequence Library for Fine-Tuning Gene Expression Levels in Saccharomyces cerevisiae.
- Construction and Optimization of Malonyl-CoA Sensors in Saccharomyces cerevisiae by Combining Promoter Engineering Strategies
- Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae
- Stepwise Optimization of Inducible Expression System for the Functional Secretion of Horseradish Peroxidase in Saccharomyces cerevisiae.
- Overexpression of genes by stress-responsive promoters increases protein secretion in Saccharomyces cerevisiae
- Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins
- Diffusion-Based Generative Network for de Novo Synthetic Promoter Design.
- From natural to synthetic: Promoter engineering in yeast expression systems.
- Tuning fungal promoters for the expression of eukaryotic proteins
- Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris
- Engineering Compartmentalization and Cofactor Regulation for 10-Hydroxy-2-decenoic Acid Biosynthesis in Saccharomyces cerevisiae.
- Advances in Fungal Promoter Engineering for Enhancing Secondary Metabolite Biosynthesis
- Komagataella phaffii as a microbial cell factory for antimicrobial peptide production.
- Synthetic Biology Strategies for Activating Cryptic BGCs in Streptomyces: Engineering Native and Synthetic Promoters for Antibiotic Discovery
- Additional file 1 of A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae
- Additional file 1 of A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae
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