An efficient method to isolate Kupffer cells eliminating endothelial cell contamination and selective bias
Explore this paper's citation graph
Summary
It is shown that conventional flow‐cytometric strategies for identification of Kupffer cells (KCs) leads to inclusion of a significant proportion of CD31hi endothelial cells, which represent endothelium tightly adhered to remnants of KC membrane.
- Type
- article
- Published
- 2018-04-01
- Cited by
- 51
- References
- 35
- Access
- Open access
- OpenAlex
- https://openalex.org/W2796050533
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4566437
Keywords
Biology, Flow cytometry, Cell sorting, Endothelial stem cell, CD31
References
- Genetic lineage tracing identifies endocardial origin of liver vasculature
- Scavenger receptors on liver Kupffer cells mediate the in vivo uptake of oxidatively damaged red blood cells in mice.
- Inhibition of Granulomatous Inflammation and Prophylactic Treatment of Schistosomiasis with a Combination of Edelfosine and Praziquantel
- Kupffer Cells in the Liver
- Cytokine-secreting follicular T cells shape the antibody repertoire
- Liver inflammation abrogates immunological tolerance induced by Kupffer cells
- Infiltrating Monocyte-Derived Macrophages and Resident Kupffer Cells Display Different Ontogeny and Functions in Acute Liver Injury
- Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis
- Liver sinusoidal endothelial cell progenitor cells promote liver regeneration in rats.
- Isolation of murine intrahepatic immune cells employing a modified procedure for mechanical disruption and functional characterization of the B, T and natural killer T cells obtained
- A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells
- CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver
- Tissue-Specific Signals Control Reversible Program of Localization and Functional Polarization of Macrophages
- Ly6Chigh Monocytes Become Alternatively Activated Macrophages in Schistosome Granulomas with Help from CD4+ Cells
- Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor
- The origin, kinetics, and characteristics of the kupffer cells in the normal steady state
- A global double‐fluorescent Cre reporter mouse
- CD11c depletion severely disrupts Th2 induction and development in vivo
- Tissue-Resident Macrophage Enhancer Landscapes Are Shaped by the Local Microenvironment
- Distinct development and functions of resident and recruited liver Kupffer cells/macrophages
Cited by
- The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages
- Recruitment of hepatic macrophages from monocytes is independent of IL‐4Rα but is associated with ablation of resident macrophages in schistosomiasis
- PIKfyve Deficiency in Myeloid Cells Impairs Lysosomal Homeostasis in Macrophages and Promotes Systemic Inflammation in Mice
- Fetal origin confers radioresistance on liver macrophages via p21cip1/WAF1
- Crosstalk network among multiple inflammatory mediators in liver fibrosis
- Mice depleted for Exchange Proteins Directly Activated by cAMP (Epac) exhibit irregular liver regeneration in response to partial hepatectomy
- Kupffer Cell Release of Platelet Activating Factor Drives Dose Limiting Toxicities of Nucleic Acid Nanocarriers.
- Transcriptional network analysis of transcriptomic diversity in resident tissue macrophages and dendritic cells in the mouse mononuclear phagocyte system
- Network analysis of transcriptomic diversity amongst resident tissue macrophages and dendritic cells in the mouse mononuclear phagocyte system
- Commensal-driven Immune Zonation of the Liver Promotes Host Defense
- Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages
- Transcriptomic Analysis of Rat Macrophages
- Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
- Fragmentation of macrophages during isolation confounds analysis of single cell preparations from mouse hematopoietic tissues
- Exercise of high intensity ameliorates hepatic inflammation and the progression of NASH
- Role of macrophages and phagocytes in orchestrating normal and pathological haematopoietic niches.
- Oral delivery of a Lactococcus lactis expressing extracellular TGFβR2 alleviates hepatic fibrosis
- A subset of Kupffer cells regulates metabolism through the expression of CD36.
- Fat-1 Transgenic Mice With Augmented n3-Polyunsaturated Fatty Acids Are Protected From Liver Injury Caused by Acute-On-Chronic Ethanol Administration
- Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation
Related papers
- Endothelial EphrinB2 Is Controlled by Microenvironmental Determinants and Associates Context-Dependently With CD31
- Isolation, purification, and conditional immortalization of murine glomerular endothelial cells of microvascular phenotype
- IN VITRO DIFFERENTIATION OF ENDOTHELIAL PROGENITOR CELL FROM RAT PERIPHERAL BLOOD
- Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.
- An Improved and Reliable Method for Isolation of Microvascular Endothelial Cells from Human Omentum
- Endothelial characteristics in healthy endothelial colony forming cells; generating a robust and valid ex vivo model for vascular disease
- Electron Microscopic Observation of the Process of Protecting Damaged Endothelium—Interaction between the Damaged Endothelium, Adjacent Endothelium, Smooth Muscle Cells, and Leucocytes: ―内皮細胞, 平滑筋細胞および白血球の関与―
- Using fluorescence activated cell sorting to examine cell-type-specific gene expression in rat brain tissue.