Determination of H+-ATPase Activity in Arabidopsis Guard Cell Protoplasts through H+-pumping Measurement and H+-ATPase Quantification.
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Summary
This protocol describes the procedures required to prepare a large amount of guard cell protoplasts and measure H-pumping by guard cells in response to blue light and fusicoccin to evaluate the activation of the H+-ATPase in vivo.
- Type
- article
- Published
- 2017-12-20
- Cited by
- 5
- References
- 16
- Access
- Open access
- OpenAlex
- https://openalex.org/W2779961813
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:89835700
Keywords
Guard cell, Fusicoccin, Protoplast, ATPase, Arabidopsis
References
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
- Blue light activates the plasma membrane H+‐ATPase by phosphorylation of the C‐terminus in stomatal guard cells
- Blue light-dependent proton extrusion by guard-cell protoplasts of Vicia faba
- Blue light activates electrogenic ion pumping in guard cell protoplasts of Vicia faba
- Biochemical characterization of plasma membrane H+-ATPase activation in guard cell protoplasts of Arabidopsis thaliana in response to blue light.
- The Plasma Membrane H+-ATPase AHA1 Plays a Major Role in Stomatal Opening in Response to Blue Light1
Cited by
- Trichoderma viride Tv-1511 Colonizes Arabidopsis Leaves and Promotes Arabidopsis Growth by Modulating the MAP Kinase 6-Mediated Activation of Plasma Membrane H+-ATPase
- Guard Cell Starch Degradation Yields Glucose for Rapid Stomatal Opening in Arabidopsis[CC-BY]
- Modulation of Ion Transport Across Plant Membranes by Polyamines: Understanding Specific Modes of Action Under Stress
- Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening
- Histone acetyltransferase GCN5-mediated lysine acetylation modulates salt stress aadaption of Trichoderma
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