Determination of H+-ATPase Activity in Arabidopsis Guard Cell Protoplasts through H+-pumping Measurement and H+-ATPase Quantification.

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Summary

This protocol describes the procedures required to prepare a large amount of guard cell protoplasts and measure H-pumping by guard cells in response to blue light and fusicoccin to evaluate the activation of the H+-ATPase in vivo.

Type
article
Published
2017-12-20
Cited by
5
References
16
Access
Open access

Keywords

Guard cell, Fusicoccin, Protoplast, ATPase, Arabidopsis

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