CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish.
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Summary
This chapter summarizes the application of CRISPR/Cas9-mediated knock-in technology in zebrafish and indicates that HR facilitates homology-dependent integration of donor DNA template into a targeted locus.
- Type
- article
- Published
- 2017-01-01
- Cited by
- 2
- References
- 16
- OpenAlex
- https://openalex.org/W2646003346
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4028285
Keywords
CRISPR, Genome editing, Cas9, Biology, Transcription activator-like effector nuclease
References
- A genetic screen for mutations affecting embryogenesis in zebrafish.
- Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering
- TALEN-mediated precise genome modification by homologous recombination in zebrafish
- Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
- Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
- Organogenesis--Heart and Blood Formation from the Zebrafish Point of View
- Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
- Genome editing using artificial site‐specific nucleases in zebrafish
- The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors
- Efficient homologous recombination-mediated genome engineering in zebrafish using TALE nucleases
- Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair
- The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio.
- Targeted mutagenesis using CRISPR/Cas system in medaka
- Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system
- MMEJ repair of double-strand breaks (director’s cut): deleted sequences and alternative endings
- Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish
Cited by
- A comparison of behavioral and reproductive parameters between wild-type, transgenic and mutant zebrafish: Could they all be considered the same "zebrafish" for reglementary assays on endocrine disruption?
- Generation of Zebrafish Models of Human Retinitis Pigmentosa Diseases Using CRISPR/Cas9-Mediated Gene Editing System
- Establishment of FUT8 gene knockout CHO cell line with stable expression of monoclonal antibody
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