Combining bleach and mild predigestion improves ancient DNA recovery from bones
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Summary
Combined together, bleach wash and predigestion consistently yield DNA libraries with higher endogenous content than either of these methods alone, and the molecular complexity of these libraries is improved and endogenous DNA templates show larger size distributions.
- Type
- article
- Published
- 2017-07-01
- Cited by
- 107
- References
- 59
- Access
- Open access
- OpenAlex
- https://openalex.org/W2540953105
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:13764564
Keywords
Bleach, DNA, Biology, DNA sequencing, Ancient DNA
References
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- Improving access to endogenous DNA in ancient bones and teeth
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- More on contamination: the use of asymmetric molecular behavior to identify authentic ancient human DNA.
- Ancient DNA: Do It Right or Not at All
- Genetic history of an archaic hominin group from Denisova Cave in Siberia
- Corrigendum: Decoding tumour phenotype by noninvasive imaging using a quantitative radiomics approach
- Use of bleach to eliminate contaminating DNA from the surface of bones and teeth.
- Predicting the molecular complexity of sequencing libraries
- Cross-biome metagenomic analyses of soil microbial communities and their functional attributes
- Assessing ancient DNA studies.
- Fragmentation of Contaminant and Endogenous DNA in Ancient Samples Determined by Shotgun Sequencing; Prospects for Human Palaeogenomics
- Genome sequence, comparative analysis and population genetics of the domestic horse (Equus caballus)
- Improving the performance of true single molecule sequencing for ancient DNA
- A bone sample cleaning method using trypsin for the isolation of DNA.
Cited by
- Ancient DNA reveals the Arctic origin of Viking Age cod from Haithabu, Germany
- The Zhenya Mammoth (Mammuthus primigenius (Blum.)): Taphonomy, geology, age, morphology and ancient DNA of a 48,000 year old frozen mummy from western Taimyr, Russia
- Comparison of two ancient DNA extraction protocols for skeletal remains from tropical environments
- Improved recovery of ancient DNA from subfossil wood - application to the world's oldest Late Glacial pine forest.
- Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
- A stainless-steel mortar, pestle and sleeve design for the efficient fragmentation of ancient bone
- Forensic DNA Analysis.
- Sexing Viking Age horses from burial and non-burial sites in Iceland using ancient DNA
- The multiple maternal legacy of the Late Iron Age group of Urville-Nacqueville (France, Normandy) documents a long-standing genetic contact zone in northwestern France
- An environmental (pre)history of European fishing: past and future archaeological contributions to sustainable fisheries.
- Isolating the human cochlea to generate bone powder for ancient DNA analysis
- Paleogenomics: reconstruction of plant evolutionary trajectories from modern and ancient DNA
- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Predigestion Step.
- Unlocking the potential of ancient fish DNA in the genomic era
- Ancient DNA reveals the chronology of walrus ivory trade from Norse Greenland
- Identifying Archaeological Bone via Non-Destructive ZooMS and the Materiality of Symbolic Expression: Examples from Iroquoian Bone Points
- Disappearance of Icelandic Walruses Coincided with Norse Settlement
- Balancing analytical goals and anthropological stewardship in the midst of the paleogenomics revolution
- The case of an arctic wild ass highlights the utility of ancient DNA for validating problematic identifications in museum collections
- A paleogenetic perspective of the Sabana de Bogotá (Northern South America) population history over the Holocene (9000 – 550 cal BP)
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