Three-dimensional CTF correction improves the resolution of electron tomograms.
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Summary
This work presents a comprehensive solution for a 3D CTF correction based on the Jensen-Kornberg scheme, which performs a slice-by-slice correction of the CTF within the tomographic reconstruction and finds that 3DCTF correction always produces equal or better results than 2D C TF correction.
- Type
- article
- Published
- 2017-02-01
- Cited by
- 42
- References
- 21
- OpenAlex
- https://openalex.org/W2466664260
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:3899284
Keywords
Contrast transfer function, Tomography, Resolution (logic), Tomographic reconstruction, Focus (optics)
References
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- Super-sampling SART with ordered subsets.
- CTF Challenge: Result Summary
- High-resolution electron microscopy of helical specimens: a fresh look at tobacco mosaic virus.
- Fast and accurate reference-free alignment of subtomograms.
- Fully Three-Dimensional Defocus-Gradient Corrected Backprojection in Cryoelectron Microscopy
- Cryo-electron microscopy of vitrified specimens
- Measurement and compensation of defocusing and aberrations by Fourier processing of electron micrographs
- A fast algorithm for computing and correcting the CTF for tilted, thick specimens in TEM.
- FASTDEF: fast defocus and astigmatism estimation for high-throughput transmission electron microscopy.
- Defocus-gradient corrected back-projection.
- A brief look at imaging and contrast transfer
- CTF Determination and Correction for Low Dose Tomographic Tilt Series
- Structure of the native Sec61 protein-conducting channel
Cited by
- Fine details in complex environments: the power of cryo-electron tomography
- Structure of RNA polymerase I transcribing ribosomal DNA genes
- The advent of structural biology in situ by single particle cryo-electron tomography
- Structural characterization of the NAP; the major adhesion complex of the human pathogen Mycoplasma genitalium
- Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4 Å
- Cryo-tomography Tilt-series Alignment with Consideration of the Beam-induced Sample Motion
- High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly.
- Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
- Locating macromolecules and determining structures inside bacterial cells using electron cryotomography
- emClarity: Software for High Resolution Cryo-electron Tomography and Sub-tomogram Averaging
- Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging.
- Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes
- Integrative biology of native cell extracts: a new era for structural characterization of life processes
- Subtomogram averaging from cryo-electron tomograms.
- In situ structure determination by subtomogram averaging
- Nucleosome conformational variability in solution and in interphase nuclei evidenced by cryo-electron microscopy of vitreous sections
- Structural analysis of pleomorphic and asymmetric viruses using cryo-electron tomography and subtomogram averaging.
- Real-time cryo–EM data pre-processing with Warp
- Subnanometer-resolution structure determination in situ by a hybrid subtomogram averaging – single particle cryoEM – workflow
- Structure and mechanism of the Nap adhesion complex from the human pathogen Mycoplasma genitalium
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