Generation of induced pluripotent stem cells.

Summary

This chapter provides the researcher with some background on iPSCs and details on how to produce MEF-conditioned medium, prepare mitotically arrested mouse embryonic fibroblasts (MEFs), createiPSCs using viral vectors, passage iPSC, and cryopreserve iPSCS.

Keywords

Induced pluripotent stem cell, Reprogramming, Embryonic stem cell, Somatic cell, Biology

References

Manipulating the mouse embryo: A laboratory manualBrigid Hogan, Frank Costantini, Elizabeth Lacy — 1986
Feeder-free growth of undifferentiated human embryonic stem cellsChunhui Xu, Margaret Inokuma, Jerrod Denham — 2001
Transduction of human embryonic stem cells by foamy virus vectors.Helen Gharwan, Roli K. Hirata, Peirong Wang — 2007
Induced Pluripotent Stem Cell Lines Derived from Human Somatic CellsJunying Yu, Maxim A. Vodyanik, Kim Smuga-Otto — 2007
Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells.Tomohisa Seki, Shinsuke Yuasa, Mayumi Oda — 2010
Controlled-rate freezing of human ES cells.Carol B. Ware, Angelique M. Nelson, C. Anthony Blau — 2005
Normal collagen and bone production by gene-targeted human osteogenesis imperfecta iPSCs.David R. Deyle, Iram Khan, Gaoying Ren — 2012
Induction of pluripotent stem cells from adult human fibroblasts by defined factors.Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki — 2007
Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cellsElayne M. Chan, Sutheera Ratanasirintrawoot, In‐Hyun Park — 2009
Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genomeNoemi Fusaki, Hiroshi Ban, Akiyo Nishiyama — 2009
The promise of induced pluripotent stem cells in research and therapyDaisy A. Robinton, George Q. Daley — 2012
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.Kazutoshi Takahashi, Shinya Yamanaka — 2006
A ROCK inhibitor permits survival of dissociated human embryonic stem cellsKiichi Watanabe, Morio Ueno, Daisuke KAMIYA — 2007

Cited by

Perspectives in Regenerative MedicineEna Ray Banerjee — 2014
Acquisition of pluripotency through continued environmental influence on OCT4-induced plastic human fibroblasts.Kyle R. Salci, Jung Bok Lee, Ryan R. Mitchell — 2015
Reprogramming Sertoli Cells into Pluripotent Stem CellsHongyan Sun, Guomin Zhang, Fulu Dong — 2014
Transduction of Oct6 or Oct9 gene concomitant with Myc family gene induced osteoblast-like phenotypic conversion in normal human fibroblasts.Naoki Mizoshiri, T Kishida, Kenta Yamamoto — 2015
Global transcriptional analysis of nuclear reprogramming in the transition from MEFs to iPSCsFulu Dong, Zhenwei Song, Jinping Zhang — 2013
Direct Delivery of Functional Proteins and Enzymes to the Cytosol Using Nanoparticle-Stabilized NanocapsulesRui Tang, Chang Soo Kim, David J. Solfiell — 2013
In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka FactorsAçelya Yılmazer, Irene de Lázaro, Cyrill Bussy — 2013
Cellular strategies for retinal repair by photoreceptor replacement.Sujatha A. Jayakody, Anai Gonzalez-Cordero, Robin R. Ali — 2015
iPSC for Dental Tissue RegenerationKim Hynes, Stan Gronthos, P. Mark Bartold — 2014
A promising approach to iPSC-based cell therapy for diabetic wound treatment: direct lineage reprogramming.Shuang Li, Qin Li — 2014
Inducing pluripotency in vitro: recent advances and highlights in induced pluripotent stem cells generation and pluripotency reprogrammingI. K. Rony, Abdul Baten, Justin A. Bloomfield — 2015
The generation of induced pluripotent stem cells from a patient with KCNH2 G603D, without LQT2 disease associated symptom.Shinichiro Okata, Shinsuke Yuasa, Teiichi Yamane — 2013
MicroRNA-22 Regulates Smooth Muscle Cell Differentiation From Stem Cells by Targeting Methyl CpG–Binding Protein 2Hanqing Zhao, Guanmei Wen, Yuan Huang — 2014
Effects of TET1 knockdown on gene expression and DNA methylation in porcine induced pluripotent stem cells.Anran Fan, Kuiying Ma, Xinglan An — 2013
The Effect of Fetal Bovine Serum (FBS) on Efficacy of Cellular Reprogramming for Induced Pluripotent Stem Cell (iPSC) GenerationDaekee Kwon, Jin‐Su Kim, Byung-Hyun Cha — 2016
Stem Cells and NanomaterialsMarie‐Claude Hofmann — 2014
ALS Astrocytes Adopt Natural Killer Properties to Induce Motor Neuron DeathSungWon Song — 2014
In vivo cell reprogramming to pluripotency : generating induced pluripotent stem cells in situ for tissue regenerationI. Rey — 2015
Stem Cells in Modeling Human Genetic DiseasesMayana Zatz, Oswaldo Keith Okamoto — 2015
Reprogramming of human cancer cells towards induced pluripotency as a tool to study the influence of epigenetic modifications on thetumor phenotypeMathias Bernhardt — 2015

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