Isolation of Peptide aptamers to target protein function.
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Summary
High-throughput protocols that facilitate the selection and characterization of peptide aptamers from yeast dihybrid libraries are described, including the preparation and evaluation of the bait fusion and the peptideaptamer screen.
- Type
- article
- Published
- 2009-01-01
- Cited by
- 8
- References
- 21
- OpenAlex
- https://openalex.org/W2395987758
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:45952845
Keywords
Aptamer, Peptide, Systematic evolution of ligands by exponential enrichment, Computational biology, Biology
References
- Escherichia coli endA deletion strain for use in two-hybrid shuttle vector selection.
- Interaction mating methods in two-hybrid systems.
- Selection of genetic agents from random peptide aptamer expression libraries.
- Fused protein domains inhibit DNA binding by LexA
- Five SWI genes are required for expression of the HO gene in yeast.
- High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
- Induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral E6 oncoprotein.
- A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
- High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
- Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
- Expression of Thioredoxin Random Peptide Libraries on the Escherichia coli Cell Surface as Functional Fusions to Flagellin: A System Designed for Exploring Protein-Protein Interactions
- Comment on “the prickly pear”
- Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2
- Selection and characterization of large collections of peptide aptamers through optimized yeast two-hybrid procedures
- Peptide aptamers: tools for biology and drug discovery.
- Correlation of two-hybrid affinity data with in vitro measurements
- Peptide Aptamers That Bind to a Geminivirus Replication Protein Interfere with Viral Replication in Plant Cells
- Targeting cyclin-dependent kinases in Drosophila with peptide aptamers.
- Yeast Two-Hybrid: State of the Art
- "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections.
Cited by
- GCN-B is a novel nuclear histone acetyltransferase that is crucial for viability in the protozoan parasite Toxoplasma gondii
- Peptide Aptamers That Bind to Geminivirus Replication Proteins Confer a Resistance Phenotype to Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus Infection in Tomato
- Peptide aptamers with biological and therapeutic applications.
- Inhibition of Human Cytomegalovirus Replication via Peptide Aptamers Directed against the Nonconventional Nuclear Localization Signal of the Essential Viral Replication Factor pUL84
- Further Characterization of the Rep Protein and Using Peptide Aptamers as a Broad-based Resistance Strategy to Combat Geminivirus Disease
- Engineering crops for resistance to geminiviruses
- Anti‐HCMV Compounds
- Tomato Infection in Tomato Mottle Virus and Virus Tomato Yellow Leaf Curl Phenotype to Replication Proteins Confer a Resistance Peptide Aptamers That Bind to Geminivirus
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