Enhancing Mn(II)-binding and manganese peroxidase activity in a designed cytochrome c peroxidase through fine-tuning secondary sphere interactions
Explore this paper's citation graph
Summary
Three mutations in the second coordination shell of an engineered Mn(II)-binding site in cytochrome c peroxidase are identified and offered molecular insight into the role of noncovalent interactions around metal-binding sites for improving metal binding and overall activity.
- Type
- article
- Published
- 2016-03-02
- Cited by
- 29
- References
- 73
- Access
- Open access
- OpenAlex
- https://openalex.org/W2283136192
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:206514614
Keywords
Chemistry, Peroxidase, Manganese, Stereochemistry, Enzyme kinetics
References
- Identifying the Elusive Sites of Tyrosyl Radicals in Cytochrome c Peroxidase: Implications for Oxidation of Substrates Bound at a Site Remote from the Heme
- The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution.
- Role of arginine 177 in the MnII binding site of manganese peroxidase. Studies with R177D, R177E, R177N, and R177Q mutants.
- Catalytic Transformation of Lignin for the Production of Chemicals and Fuels.
- Crystal structure of guaiacol and phenol bound to a heme peroxidase
- Crystal structure of yeast cytochrome c peroxidase refined at 1.7-A resolution.
- Effects of pH and Temperature on Recombinant Manganese Peroxidase Production and Stability
- Manganese oxidation site in Pleurotus eryngii versatile peroxidase: a site-directed mutagenesis, kinetic, and crystallographic study.
- Expression, purification, characterization, and solution nuclear magnetic resonance study of highly deuterated yeast cytochrome C peroxidase with enhanced solubility.
- Comparison of simple potential functions for simulating liquid water
- Effects of buried ionizable amino acids on the reduction potential of recombinant myoglobin.
- A spectrophotometric method for the simultaneous determination of myoglobin and hemoglobin in extracts of human muscle.
- Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus.
- Reaction pathway analysis of thermal and catalytic lignin fragmentation by use of model compounds
- Redesign of cytochrome c peroxidase into a manganese peroxidase: role of tryptophans in peroxidase activity.
- Expression of a Phanerochaete chrysosporium Manganese Peroxidase Gene in the Yeast Pichia pastoris
- Fungal biodegradation and enzymatic modification of lignin.
- Stimulation of Mn peroxidase activity: a possible role for oxalate in lignin biodegradation.
- Rationally Tuning the Reduction Potential of a Single Cupredoxin Beyond the Natural Range
- Construction and characterization of a manganese-binding site in cytochrome c peroxidase: towards a novel manganese peroxidase.
Cited by
- Design and Engineering of Artificial Oxygen-Activating Metalloenzymes
- Design of Heteronuclear Metalloenzymes
- Rational design of metalloenzymes: From single to multiple active sites
- Using an artificial tryptophan "wire" in cytochrome c peroxidase for oxidation of organic substrates.
- Biological methods for textile dye removal from wastewater: A review
- An Efficient, Step-Economical Strategy for the Design of Functional Metalloproteins
- Enzymatic and Bacterial modifications of natural Biofibres for Synthesis of biocomposites
- An evolutionary path to altered cofactor specificity in a metalloenzyme
- A Binuclear CuA Center Designed in an All α-Helical Protein Scaffold
- Contributions of primary coordination ligands and importance of outer sphere interactions in UFsc, a de novo designed protein with high affinity for metal ions.
- Effects of stabilizers on sustainability, activity and decolorization performance of Manganese Peroxidase enzyme produced by Phanerochaete chrysosporium
- Biodegradation of aromatic pollutants by metalloenzymes: A structural-functional-environmental perspective
- Extremophilic Ligninolytic Enzymes: Versatile Biocatalytic Tools with Impressive Biotechnological Potential
- The second-shell amino acid R266 helps determine N-succinylamino acid racemase reaction specificity in promiscuous N-succinylamino acid racemase/o-succinylbenzoate synthase enzymes
- Artificial metalloenzymes based on protein assembly
- Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere
- Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440.
- Biological approaches to the purification of textile wastewater
- Structural Basis for the Effects of Phenylalanine on Tuning the Reduction Potential of Type 1 Copper in Azurin.
- A designed Copper Histidine-brace enzyme for oxidative depolymerization of polysaccharides as a model of lytic polysaccharide monooxygenase
Related papers
- Plant peroxidases. Their primary, secondary and tertiary structures, and relation to cytochrome c peroxidase.
- Assignment of hyperfine shifted resonances in high-spin forms of cytochrome c peroxidase by reconstitutions with deuterated hemins.
- Two extracellular proteins with alkaline peroxidase activity, a novel cytochrome c and a catalase-peroxidase, from Bacillus sp. No.13
- Crystal structure of the ascorbate peroxidase–ascorbate complex
- Cytochrome c as a Peroxidase: Activation of the Precatalytic Native State by H2O2-Induced Covalent Modifications.
- Bacterial catalase-peroxidases are gene duplicated members of the plant peroxidase superfamily.
- Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plants
- Thirty Years of Heme Peroxidase Structural Biology
- Crystallographic and FTIR spectroscopic evidence of changes in Fe coordination upon reduction of the active site of the Fe-only hydrogenase from Desulfovibrio desulfuricans.
- Defining substrate specificity and catalytic mechanism in ascorbate peroxidase.