The bradford method for protein quantitation.
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Summary
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study, but is susceptible to interference from a wide range of compounds commonly present in biological extracts.
- Type
- article
- Published
- 1988-01-01
- Cited by
- 2,102
- References
- 13
- OpenAlex
- https://openalex.org/W2244276231
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:31851660
Keywords
Lowry protein assay, Bradford protein assay, Bicinchoninic acid assay, Chromatography, Reagent
References
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
- Interference by lipids in the determination of protein using bicinchoninic acid.
- Adaptation of the dye-binding protein assay to microtiter plates.
- Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.
- A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.
- Studies and critique of Amido Black 10B, Coomassie Blue R, and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis.
- Sensitivity and variability of the Bradford protein assay in the presence of detergents.
- Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents.
- Mechanism of dye response and interference in the Bradford protein assay.
- Assay for protein by dye binding.
- Increased uniformity in the response of the coomassie blue G protein assay to different proteins.
- Measurement of protein using bicinchoninic acid.
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