Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
Explore this paper's citation graph
Summary
A rapid method for the flow microfluorometric determination of the DNA content per cell is described, which requires a minimal amount of material, and avoids formation of cell clumps.
- Type
- article
- Published
- 1975-07-01
- Cited by
- 1,794
- References
- 10
- Access
- Open access
- OpenAlex
- https://openalex.org/W2169790726
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16772789
Keywords
Propidium iodide, Biology, Staining, Cell, Cell cycle
References
- Use of flow microfluorometry in detailed analysis of effects of chemical agents on cell cycle progression.
- A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.
- Microfluorimetric evaluation of cell kill kinetics with 1-beta-D-arabinofuranosylcytosine.
- 2,3-Dihydro-1H-imidazo(1,2-b)pyrazole: a new inhibitor of deoxyribonucleic acid synthesis.
- The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.
- Cell-Cycle Analysis in 20 Minutes
- RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN CELL POPULATIONS
- Cell Microfluorometry: A Method for Rapid Fluorescence Measurement
- Adaptation of the fluorescent Feulgen reaction to cells in suspension for flow microfluorometry.
Cited by
- Recurrent renal cell carcinoma arising in wilms' tumor
- Accurate quantitation of the low number of white cells in white cell‐depleted blood components
- Dual-parameter flow cytometric analysis of an early lymphocyte activation antigen (CK226) and DNA content.
- Tumor acquisition, propagation, and preservation. The culture of human colorectal cancer
- Down's syndrome and acute myelofibrosis. Time study of dna content during the progression to leukemia
- Allocation of the suppressive activity of normal peripheral blood lymphocytes induced by diffusion chamber culture and Con A stimulation to the G2 phase of the cell cycle.
- An antigen receptor-driven, interleukin 2-independent pathway for proliferation of murine cytolytic T lymphocyte clones
- Different effect of thymidine kinase loss on TTP pools; comparison among human leukemia cell lines.
- Expression of messenger ribonucleic acid for epidermal growth factor (EGF), transforming growth factor-alpha (TGF alpha), and EGF receptor in human amnion cells: possible role of TGF alpha in prostaglandin E2 synthesis and cell proliferation.
- DNA flow cytometric evaluation of cell cycle distribution in ulcerative colitis: a proposed method for assessing severity of disease.
- Studies on signal transduction mechanisms for adrenaline-driven lipolysis in white and brown adipocytes.
- Endothelial cell replication in an in vivo model of aortic allografts.
- Eosinophil viability during immunoglobulin‐induced degranulation
- Distinct effects of ceramide‐generating pathways in prostate adenocarcinoma cells
- Flow cytometric analysis of megakaryocyte ploidy. Comparison with Feulgen microdensitometry and discovery that 8N is the predominant ploidy class in guinea pig and monkey marrow.
- Flow Cytometric Analysis of DNA Content of Living and Fixed Cells: a Comparative Study Using Various Fixatives
- Membrane-targeted green fluorescent protein reliably and uniquely marks cells through apoptotic death.
- Flow cytometric analysis of normal human megakaryocytes.
- Analysis of neural potential of human umbilical cord blood–derived multipotent mesenchymal stem cells in response to a range of neurogenic stimuli
- Genetic and epigenetic inactivation of T‐cadherin in human hepatocellular carcinoma cells
Related papers
- Application of three kinds of staining methods for protein in SDS-PAGE
- Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.
- Effect of adriamycin and analogs on the nuclear fluorescence of propidium iodide-stained cells.
- A modified Hemetoxylin and Eosin Staining method
- Comparison of Three Staining Techniques for S180 Ascites Smear Observation
- A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining.
- Study on Comparison of Methods for Detection of Cell Apoptosis by Flow Cytometry
- A Pipeline for Developing and Testing Staining Protocols for Flow Cytometry, Demonstrated with SYBR Green I and Propidium Iodide Viability Staining
- Flow cytometry as a method for studying effects of stressors on primary rat neurons
- A versatile flow cytometry-based assay for the determination of short- and long-term natural killer cell activity.