Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
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Summary
This work characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously and applies this method to sequencing a human genome and finds that coverage biases are comparable to those of conventional protocols.
- Type
- article
- Published
- 2010-12-08
- Cited by
- 562
- References
- 26
- Access
- Open access
- OpenAlex
- https://openalex.org/W2164643779
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:18229352
Keywords
Transposase, Shotgun, Shotgun sequencing, Computational biology, Transposition (logic)
References
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- Next-generation DNA sequencing
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- Tn5/IS50 target recognition.
- What would you do if you could sequence everything?
- TnphoA: a transposon probe for protein export signals.
- The Sequence Alignment/Map format and SAMtools
- A general method for isolation of high molecular weight DNA from eukaryotes.
- Genome sequencing in microfabricated high-density picolitre reactors
- Tn5 as a model for understanding DNA transposition
- Real-Time DNA Sequencing from Single Polymerase Molecules
- Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding.
- Sequencing of Fifty Human Exomes Reveals Adaptation to High Altitude
- Mapping and quantifying mammalian transcriptomes by RNA-Seq
Cited by
- SNES: single nucleus exome sequencing
- Impact of library preparation protocols and template quantity on the metagenomic reconstruction of a mock microbial community
- A brief review of single-cell transcriptomic technologies.
- Advances in DNA Detection
- qPCR-based characterization of DNA fragmentation efficiency of Tn5 transposomes
- Preparation of metagenomic libraries from naturally occurring marine viruses.
- VALIDATING APPROACHES FOR STUDYING MICROBIAL DIVERSITY TO CHARACTERIZE COMMUNITIES FROM ROOTS OF Populus deltoides
- Genetics of congenital heart disease: the contribution of the noncoding regulatory genome
- Development of a comprehensive massively parallel sequencing panel of single nucleotide polymorphism and short tandem repeat markers for human identification
- Taking Stock of Regulatory Variation.
- Multiplex Single Cell Profiling of Chromatin Accessibility by Combinatorial Cellular Indexing
- Overview Article: Identifying transcriptional cis-regulatory modules in animal genomes
- The future is now: single-cell genomics of bacteria and archaea
- ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide
- Evolutionary engineering of a wine yeast strain revealed a key role of inositol and mannoprotein metabolism during low-temperature fermentation
- Exome Sequencing by Targeted Enrichment
- Single-cell chromatin accessibility reveals principles of regulatory variation
- An integrated genotyping approach for HLA and other complex genetic systems.
- Diagnostic applications of next generation sequencing: working towards quality standards/Diagnostische Anwendung von Next Generation Sequencing: Auf dem Weg zu Qualitätsstandards
- Novel Y-chromosome Short Tandem Repeat Variants Detected Through the Use of Massively Parallel Sequencing
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