piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
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Summary
It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.
- Type
- article
- Published
- 2009-03-01
- Cited by
- 1,890
- References
- 33
- Access
- Open access
- OpenAlex
- https://openalex.org/W2158979552
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16814541
Keywords
Reprogramming, Induced pluripotent stem cell, Biology, SOX2, Transposase
References
- Manipulating the mouse embryo: A laboratory manual
- Manipulating the Mouse Embryo: A Laboratory Manual
- Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.
- Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction
- Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses.
- PiggyBac transposon-mediated gene transfer in human cells.
- Transposon-free insertions for insect genetic engineering
- Induced pluripotent stem cells generated without viral integration
- Second‐generation tetracycline‐regulatable promoter: repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness
- Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
- Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors
- 17-P022 Virus-free induction of pluripotency and subsequentexcision of reprogramming factors
- Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
- Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution.
- Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage.
- Generation of germline-competent induced pluripotent stem cells
- A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types
- Induction of pluripotent stem cells from fibroblast cultures
- piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells
- The NuRD component Mbd3 is required for pluripotency of embryonic stem cells
Cited by
- Molecular and cellular basis of hematopoietic stem cells maintenance and differentiation
- Helper-independent piggyBac plasmids for gene delivery approaches: Strategies for avoiding potential genotoxic effects
- Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering
- Stem cells and spinal cord injury repair.
- Cell transplantation therapies for spinal cord injury focusing on induced pluripotent stem cells
- Stem Cell Models for Drug Discovery and Toxicology Studies
- Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells
- Gene delivery in tissue engineering and regenerative medicine.
- Subcellular localisation of the stem cell markers OCT4, SOX2, NANOG, KLF4 and c-MYC in cancer: a review
- The Differentiation Stage of Transplanted Stem Cells Modulates Nerve Regeneration
- Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching
- Development of Novel Tools for Radial Glia Lineage Tracing and Modeling Central Nervous System Tumor
- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors.
- Life before Nkx2.5: cardiovascular progenitor cells: embryonic origins and development.
- Control of embryonic stem cell fate: the role of phosphoinositide 3-kinase signalling and Zscan4
- Dynamic histone modifications at the promoters of Hox genes in embryonic stem cells
- Small molecules in cellular reprogramming and differentiation.
- Induced pluripotent stem cells (iPSc) for gene therapy.
- Biological impact of human embryonic stem cells.
- Current applications of transposons in mouse genetics.
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