A practical guide to evaluating colocalization in biological microscopy.

Keywords

Colocalization, Fluorescence microscope, Microscopy, Computational biology, Function (biology)

References

• Spatial Autocorrelation in Ecological Studies: A Legacy of Solutions and MythsMarie‐Josée Fortin, Mark R. T. Dale — 2009
• Confined displacement algorithm determines true and random colocalization in fluorescence microscopyOmar A. Ramírez, Alejandra García, Raúl Rojas — 2010
• Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells.Exing Wang, Janice Pennington, James R. Goldenring — 2001
• Assessing the significance of the correlation between two spatial processes.Peter Clifford, Sylvia Richardson, Denis Hémon — 1989
• Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative and recycling compartmentsDawei Zhang, Manoj Manna, Thorsten Wohland — 2009
• Automated organelle‐based colocalization in whole‐cell imagingBen J. Woodcroft, Luke A. Hammond, Jennifer L. Stow — 2009
• Mathematical Contributions to the Theory of Evolution. III. Regression, Heredity, and PanmixiaKarl Pearson — 1896
• Beyond co-localization: inferring spatial interactions between sub-cellular structures from microscopy imagesJo A. Helmuth, Grégory Paul, Ivo F. Sbalzarini — 2010
• Measurement of co‐localization of objects in dual‐colour confocal imagesErik M. M. Manders, Fons J. Verbeek, Jacob A. Aten — 1993
• The Elimination of Spurious Correlation due to Position in Time or SpaceStudent — 1914
• Modifying the t test for assessing the correlation between two spatial processesPierre Dutilleul, Peter Clifford, Sylvia Richardson — 1993
• A guide to accurate fluorescence microscopy colocalization measurements.Jonathan Comeau, Santiago Costantino, Paul W. Wiseman — 2006
• Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosomeKenneth W. Dunn, Timothy E. McGraw, Frederick R. Maxfield — 1989
• Replicate‐based noise corrected correlation for accurate measurements of colocalizationJeremy Adler, Stamatis N. Pagakis, Ingela Parmryd — 2008
• Targeting of the 5-HT1A Serotonin Receptor to Neuronal Dendrites Is Mediated by Yif1BDamien Carrel, Justine Masson, Sana Al Awabdh — 2008
• RhoB‐Dependent Modulation of Postendocytic Traffic in Polarized Madin‐Darby Canine Kidney CellsChristine Rondanino, Raúl Rojas, Wily G. Ruiz — 2007
• Dynamic adhesions and MARCKS in melanoma cellsAdriana Estrada‐Bernal, Jesse C. Gatlin, Somkiat Sunpaweravong — 2009
• Quantifying colocalization by correlation: The Pearson correlation coefficient is superior to the Mander's overlap coefficientJeremy Adler, Ingela Parmryd — 2010
• Image acquisition for colocalization using optical microscopy.David R.L. Scriven, Ronald M. Lynch, Edwin D.W. Moore — 2008
• An automated method to quantify and visualize colocalized fluorescent signals.Frédéric Jaskolski, Christophe Mulle, Olivier J. Manzoni — 2005

Cited by

• KSHV attachment and entry are dependent on αVβ3 integrin localized to specific cell surface microdomains and do not correlate with the presence of heparan sulfateGeorge Ritzer, Jeffrey Stepnisky, John Lemich — 2014
• Topologically Dissociable Patterns of Development of the Human Cerebral CortexS. Bertoletti, G.P. Gaspari, C. Lombardi — 2015
• CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing FactorAntonio Cortés Ruiz — 2016
• Simultaneous multicolor imaging of wide-field epi-fluorescence microscopy with four-bucket detection.Rózsa Péter — 2016
• Airway epithelial homeostasis and planar cell polarity signaling depend on multiciliated cell differentiation由紀 佐藤, 美子 千葉, 勝悦 葛岡 — 2016
• New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans.Saroj G. Regmi, S. Rolland — 2017
• On chip Cryo-Anesthesia of Drosophila Larvae for High Resolution In vivo Imaging ApplicationsFernando Calatayud — 2017
• IGF2BP1/IMP1 Deletion Enhances a Facultative Stem Cell State via Regulation of MAP1LC3BPetis László — 2023
• Biochemical and imaging methods to study receptor membrane organization and association with lipid rafts.Bruno Sotto-Mayor, Juan A. Torreño-Pina, Thomas S. van Zanten — 2013
• Atopic donor status does not influence the uptake of the major grass pollen allergen, Phl p 5, by dendritic cellsKazem Ashjaei, Dieter Palmberger, Merima Bublin — 2015
• Application of anti-LRP/LR specific antibodies for the treatment of Alzheimer's diseaseK. Jovanović — 2014
• Colocalization Estimation Using Graphical Modeling and Variational Bayesian Expectation Maximization: Towards a Parameter-Free ApproachSuyash P. Awate, Thyagarajan Radhakrishnan — 2015
• Development of target-specific liposomes for delivering small molecule drugs after reperfused myocardial infarctionSiva Sai Krishna Dasa, Ryo Suzuki, Michael Gutknecht — 2015
• Characterization of Suppressor of IKKepsilon function using immunofluorescence assays and quantitative colocalization analysisKenneth F Lawrence — 2014
• The gamma-smooth muscle actin isoform in smooth muscle and smooth muscle-like cellsR. Arnoldi — 2013
• Trafficking of the IKs‐Complex in MDCK Cells: Site of Subunit Assembly and Determinants of Polarized LocalizationJens‐Peter David, Martin N. Andersen, Søren‐Peter Olesen — 2013
• Development of Drug-loaded Nanoparticles for Targeted Chemotherapy.Teppei Shirakura — 2015
• Amyloid fibrils are nanoparticles that target lysosomes.Toral Jakhria — 2014
• Cutting Edge: Developmental Regulation of IFN-γ Production by Mouse Neutrophil Precursor CellsCarolyn R. Sturge, Elise Burger, Megan Raetz — 2015
• Subcellular localisation of retromer in post‐endocytic pathways of polarised Madin–Darby canine kidney cellsMaravillas Mellado-López, Yasmina Cuartero, Ramón Brugada — 2014

Related papers

• Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review.Karen R Steingart, Megan Henry, Vivienne Ng — 2006
• The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical MicroscopyYu Zhang, Zhilie Tang, Yongbo Wu — 2018
• [Comparative study of microscopy by the Ziehl Neelsen method, routine fluorescence microscopy, and fluorescence microscopy using a lumin attachment in the diagnosis of acid-resistant mycobacteria].A A Turusov, R Sh Valiev, R V Chesnokova — 2009
• Video intensification microscopy (VIM) as an aid in routine fluorescence microscopyDavid S. Forman, David E. Turriff — 2004
• Fundamentals of Fluorescence Microscopy: Exploring Life with LightPartha Pratim Mondal, Alberto Diaspro — 2013
• Hybrid adaptive and computational light-sheet fluorescence microscopyDean Wilding, Paolo Pozzi, Oleg Soloviev — 2018
• Selective plane illumination microscopy techniques in developmental biologyJan Huisken, Didier Y. R. Stainier — 2009
• [Fluorescence microscopy].Peter Jomo Walla — 1965