Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
Explore this paper's citation graph
Summary
An alternative method for the synthesis of specific DNA sequences is explored that involves the reciprocal interaction of two oligonucleotides and the DNA polymerase extension products whose synthesis they prime, when they are hybridized to different strands of a DNA template in a relative orientation such that their extension products overlap.
- Type
- article
- Published
- 1986-01-01
- Cited by
- 3,575
- References
- 14
- OpenAlex
- https://openalex.org/W2148312059
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:26180176
Keywords
Restriction enzyme, Cloning (programming), Molecular cloning, Polymerase chain reaction, DNA
References
- Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
- Chemical synthesis and expression in yeast of a gene encoding connective tissue activating peptide-III. A novel approach for the facile assembly of a gene encoding a human platelet-derived mitogen.
- An alternate method for synthesis of double-stranded DNA segments.
- Construction of biologically functional bacterial plasmids in vitro.
- Synthetic Gene Fragments in Genetic Engineering—The Renaissance of Chemistry in Molecular Biology
- Homology requirements for recombination in Escherichia coli.
- Base modification and cloning efficiency of oligodeoxyribonucleotides synthesized by the phosphoramidite method: methyl versus cyanoethyl phosphorus protection.
- A restriction enzyme from Hemophilus influenzae. II.
Cited by
- In vitro recombination and mutagenesis by overlap extension PCR.
- The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site.
- DNA-Based Techniques in Clinical Biochemistry: A Beginner's Guide to Theory and Practice
- Strategies for the genetic manipulation of Saccharomyces cerevisiae.
- Simultaneous analysis of multiple polymorphic loci using amplified sequence polymorphisms (ASPs).
- A comparative study of genetic variation at five VNTR loci in three ethnic groups of Houston, Texas.
- The efficiency of multilocus DNA fingerprint probes for individualization and establishment of family relationships, determined from extensive casework.
- Laboratory diagnostic aspects of drug resistant tuberculosis.
- The polymerase chain reaction in diagnosing lymphoid disorders
- Detection of bovine leukemia virus proviral DNA in individual cells.
- Structural characterization of the gene encoding rat 25-hydroxyvitamin D3 24-hydroxylase.
- PCR inhibition assay for DNA-targeted antibiotics.
- Direct in situ single-copy (DISC)-PCR.
- Molecular diagnostics for dairy-borne pathogens.
- The mitochondrial control region of Cervidae: evolutionary patterns and phylogenetic content.
- Identification and characterization of genes with specific expression in dendritic cells
- Real‐time Quantitative Polymerase Chain Reaction: A Potential Tool for Genetic Analysis in Neuropathology
- From PCR to RCA: a surgical trainee's guide to the techniques of genetic amplification.
- Quantitative detection of low-copy-number mRNAs differing at single nucleotide positions.
- Infection induced chronic inflammation and it's association with prostate cancer initiation and progression
Related papers
- Automatic construction of restriction site maps
- WebFARM: web server for finite automated restriction mapping
- Restriction Site Mapping of the Mitochondrial DNA of the Four-Wing Flyingfish, Hirundichthys affinis
- Using of Data Base to Determine the Restriction Sites and Drawing Restriction Map for Lipase Gene from Bacillus stearothermophilus
- Inverse restriction site mutation (iRSM) analysis. Mutation detection involving the formation of restriction enzyme sites in target genes.
- Derivation of a physical map of chloroplast DNA from Nicotiana tabacum by two-dimensional gel and computer-aided restriction analysis.
- KLONER; a computer program to simulate recombinant DNA strategies by restriction map manipulation
- Cloning DNA fragments between two adjacent/overlapping restriction sites using a "positive stuffer".