An improved method for the extraction of nematodes using iodixanol (OptiPrep
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Summary
This technique resulted in significantly higher numbers of clean eggs and vermiform nematodes that retain higher viability than samples processed with the sucrose method, with the added benefit of yielding cleaner samples compared to traditional isolation techniques.
- Type
- article
- Published
- 2008-07-31
- Cited by
- 11
- References
- 10
- Access
- Open access
- OpenAlex
- https://openalex.org/W2128451639
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:54799977
Keywords
Nematode, Extraction (chemistry), Isolation (microbiology), Iodixanol, Density gradient
References
- Extraction and processing of plant and soil nematodes.
- Effect of exsheathment on motility and pathogenicity of two entomopathogenic nematode species.
- Iodixanol: a nonionic iso-osmotic centrifugation medium for the formation of self-generated gradients.
- The use of iodixanol as a density gradient material for separating human sperm from semen.
- Efficiency of the Galleria (wax moth) baiting technique for recovering infective stages of entomopathogenic rhabditids (Steinernematidae and Heterorhabditidae) from Sand and soil
- Comparison of three methods for estimating the number of entomopathogenic nematodes present in soil samples.
- Plant parasitic nematodes in subtropical and tropical agriculture
- Laboratory Extraction Methods and Field Detection of Entomophilic Rhabditoid Nematodes From Soil
- A rapid centrifugal-flotation technique for separating nematodes from soil.
Cited by
- New methods of removing debris and high-throughput counting of cyst nematode eggs extracted from field soil
- CANONICAL DISCRIMINANT ANALYSIS OF ROTYLENCHULUS RENIFORMIS IN ALABAMA
- Characterization of the Two Intra-Individual Sequence Variants in the 18S rRNA Gene in the Plant Parasitic Nematode, Rotylenchulus reniformis
- Characterization of the reniform nematode genome by shotgun sequencing.
- Principal Component Analysis and Molecular Characterization of Reniform Nematode Populations in Alabama
- Studies on extraction of Meloidogyne incognita from tuberose (Polianthes tuberosa L.) bulbs using different techniques and its management with carbosulfan
- MOLECULAR BIOLOGY AND PHYSIOLOGY 18S and ITS1 Genomic Sequence Variations in Rotylenchulus reniformis Isolates from Alabama
- Automated image analysis systems to quantify physical and behavioral attributes of biological entities
- EFICIÊNCIA DE DIFERENTES MÉTODOS DE EXTRAÇÃO DE MELOIDOGYNE SPP. NA CULTURA DA ACEROLEIRA
- MOLECULAR BIOLOGY AND PHYSIOLOGY 18S and ITS1 Genomic Sequence Variations in Rotylenchulus reniformis Isolates from Alabama
- Reniform Nematode Morphometric Variation in Alabama : Nyaku et al .
- Genetic Resistance to the Reniform Nematode in Cotton