Modified fabrication process of protein chips using a short-chain self-assembled monolayer
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Summary
The effects of the solvents used in the washing step are studied to solve the problem of uniform fluorescent intensity in protein chips and reveal that PBST (PBS + 5% Tween20) is more effective in reducing the hydrolysis of NHS esters than deionized water.
- Type
- article
- Published
- 2008-04-01
- Cited by
- 73
- References
- 26
- OpenAlex
- https://openalex.org/W2122929169
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:31878210
Keywords
Monolayer, Hydrolysis, Fluorescence, Aqueous solution, Chemistry
References
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- Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance.
- Design and self-assembly of open, regular, 3D mesostructures
- Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions.
- Immunological activity of IgG Langmuir films oriented by protein A sublayer
- Zero-length crosslinking procedure with the use of active esters.
Cited by
- Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device
- The Study of an Ultrasensitive Label-Free Immunosensing-Chip of Quartz-Crystal Microbalance for Morphine
- The art of signal transforming: electrodes and their smart applications in electrochemical sensing
- Genome-guided insights into the versatile metabolic capabilities of the mercaptosuccinate-utilizing β-proteobacterium Variovorax paradoxus strain B4.
- Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM)
- Self-assembled monolayer for AFM measurements of Tobacco Mosaic Virus (TMV) at the atomic level
- Direct electron transfer of glucose oxidase at glassy carbon electrode modified with functionalized carbon nanotubes within a dihexadecylphosphate film
- Novel gold-decorated polyaniline derivatives as redox-active species for simultaneous detection of three biomarkers of lung cancer.
- Comparative analysis of QCM and SPR techniques for the optimization of immobilization sequences
- Recent advances in self-assembled monolayers based biomolecular electronic devices.
- Reusable biosensors via in situ electrochemical surface regeneration in microfluidic applications.
- Application of poly(amidoamine) dendrimers for use in bionanomotor systems.
- Electrochemical studies of homogeneous self-assembled monolayers versus mixed self-assembled monolayers on gold electrode for “label free” detection of heart fatty acid binding protein
- Collagen cryogel cross-linked by naturally derived dialdehyde carboxymethyl cellulose.
- Methods of reducing non-specific adsorption in microfluidic biosensors
- Simple surface chemistry to immobilize DNA probes that significantly increases sensitivity and spots density of surface plasmon resonance imaging based microarray systems
- Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes.
- Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin
- Preparation and Characterization of Covalently Binding of Rat Anti-human IgG Monolayer on Thiol-Modified Gold Surface
- New arginine substituted derivative of poly(allylamine hydrochloride) for heparin reversal
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