Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies
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Summary
The results of this study may be used as a guideline for selecting primer pairs with the best overall coverage and phylum spectrum for specific applications, therefore reducing the bias in PCR-based microbial diversity studies.
- Type
- article
- Published
- 2012-08-28
- Cited by
- 8,073
- References
- 57
- Access
- Open access
- OpenAlex
- https://openalex.org/W2115701239
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16752973
Keywords
Biology, Amplicon, 16S ribosomal RNA, Primer (cosmetics), Genetics
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- Tumour-associated and non-tumour-associated microbiota in colorectal cancer
- Improving the microbial community reconstruction at the genus level by multiple 16S rRNA regions.
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- Chitin Mixed in Potting Soil Alters Lettuce Growth, the Survival of Zoonotic Bacteria on the Leaves and Associated Rhizosphere Microbiology
- Improved metagenome assemblies and taxonomic binning using long-read circular consensus sequence data
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- High pressure activation of the Mrr restriction endonuclease in Escherichia coli involves tetramer dissociation
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- Short-chain fatty acids and inulin, but not guar gum, prevent diet-induced obesity and insulin resistance through differential mechanisms in mice
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- Unraveling the gut microbiome of the long-lived naked mole-rat
- Organic Amendments in a Long-term Field Trial—Consequences for the Bulk Soil Bacterial Community as Revealed by Network Analysis
- The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge
- Bacterial Diversity and Biogeochemistry of Two Marine Shallow-Water Hydrothermal Systems off Dominica (Lesser Antilles)
- Differentially Regulated Host Proteins Associated with Chronic Rhinosinusitis Are Correlated with the Sinonasal Microbiome
- Metagenomic study of bacterial microbiota in persistent endodontic infections using Next‐generation sequencing
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