A Highly Efficient Molecular Cloning Platform that Utilises a Small Bacterial Toxin Gene
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Summary
These engineered cloning vectors containing variants of IbsC, a 19‐residue toxin from Escherichia coli K‐12, are as reliable and amenable to high‐throughput cloning as commercially available systems, and have the advantage of being more time‐efficient and cost‐effective.
- Type
- article
- Published
- 2013-04-15
- Cited by
- 10
- References
- 24
- OpenAlex
- https://openalex.org/W2103537794
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:5269456
Keywords
Cloning (programming), Molecular cloning, Computational biology, Escherichia coli, Biology
References
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- Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
- Positive-selection vectors using the F plasmid ccdB killer gene.
- A One Pot, One Step, Precision Cloning Method with High Throughput Capability
- Abundance of type I toxin–antitoxin systems in bacteria: searches for new candidates and discovery of novel families
- Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
- Identification of a Toxic Peptide through Bidirectional Expression of Small RNAs
- Repression of small toxic protein synthesis by the Sib and OhsC small RNAs
- Decoding Toxicity
- PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.
- Construction of a novel zero background prokaryotic expression vector: potential advantages
- Separate-component-stabilization system for protein and DNA production without the use of antibiotics.
- The art of selective killing: plasmid toxin/antitoxin systems and their technological applications.
- Artificial activation of toxin-antitoxin systems as an antibacterial strategy
- Recognition and discrimination of target mRNAs by Sib RNAs, a cis-encoded sRNA family
- Molecular cloning and expression of a receptor for human tumor necrosis factor.
- Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.
- Many paths to many clones: a comparative look at high-throughput cloning methods.
Cited by
- BtoxDB: A comprehensive database of protein structural data on toxin-antitoxin systems
- IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation
- Toxin-antitoxin systems and its biotechnological applications
- Polishing the craft of genetic diversity creation in directed evolution.
- Linking bacterial type I toxins with their actions.
- Intracellular selection of trans-cleaving hammerhead ribozymes
- Intracellular Selection of Theophylline-Sensitive Hammerhead Aptazyme
- Frame-shifted proteins of a given gene retain the same function
- Biology and evolution of bacterial toxin–antitoxin systems
- Trans-acting aptazyme for conditional gene knockdown in eukaryotic cells
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