Development and mapping of microsatellite (SSR) markers in wheat

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Summary

The efficiency of marker development could be increased in wheat by creating libraries from sheared rather than enzyme-digested DNA fragments for microsatellite screening, by focusing on microsatellites with the [ATT/TAA]n motif, and by adding an untemplated G-C clamp to the 5′-end of primers.

Type
article
Published
2005-01-18
Cited by
649
References
77

Keywords

Biology, Plant biochemistry, Microsatellite, Genetic marker, Evolutionary biology

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