Hybridization of DNA to RNA in methylmercuric hydroxide agarose gels.
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Summary
This method for hybridization of cDNA probes to RNA directly in agarose gels which provides a practical alternative to methods involving transfer of the RNA out of the gel while maintaining a level of sensitivity comparable to other procedures.
- Type
- article
- Published
- 1984-07-01
- Cited by
- 1
- References
- 11
- OpenAlex
- https://openalex.org/W2074201774
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1164288
Keywords
Agarose, RNA, Agarose gel electrophoresis, Chemistry, DNA
References
- Cloning of a double-stranded cDNA that codes for a portion of chicken preproalbumin. A general method for isolating a specific DNA sequence from partially purified mRNA.
- Primary activation of the vitellogenin gene in the rooster.
- Hybridization of labeled RNA to DNA in agarose gels.
- Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
- Hybridization of nucleic acids directly in agarose gels.
- Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
- Detection of specific sequences among DNA fragments separated by gel electrophoresis.
- Isolation and characterization of a cDNA clone specific for avian vitellogenin II
- A simple and reliable method for the drying of polyacrylamide slab gels.
- The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, cloning, and evidence for an intervening sequence.
- Nucleic Acids Research Isolation and characterization of a cDNA clone specific for avian vitellogenin II
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