Intein-mediated rapid purification of Cre recombinase.

Keywords

Intein, Biology, Fusion protein, Escherichia coli, Recombinant DNA

References

• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Richard W. McEnally, Rebecca B. Todd — 1976
• Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein.Maurice W. Southworth, Kensey R. Amaya, Thomas C. Evans — 1999
• Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites.Nat Sternberg, Daniel L. Hamilton — 1981
• Rapid construction of recombinant DNA by the univector plasmid-fusion system.Qinghua Liu, Mamie Z. Li, Dou Liu — 2000
• Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.Ken Abremski, R H Hoess — 1984
• Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase.Patricia M. Kane, Carl T. Yamashiro, David F Wolczyk — 1990
• Genomic targeting with an MBP-Cre fusion protein.Andreas F. Kolb, Stuart G. Siddell — 1996
• Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.Shaorong Chong, Fana B. Mersha, Donald G. Comb — 1997
• The in Vitro Ligation of Bacterially Expressed Proteins Using an Intein from Methanobacterium thermoautotrophicum *Thomas C. Evans, Jack S. Benner, Ming‐Qun Xu — 1999
• Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature.Francine B. Perler, Elaine O. Davis, Gary E. Dean — 1994
• Gene fusion expression systems in Escherichia coli.Edward R. LaVallie, John McCoy — 1995
• Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein*Shaorong Chong, Kay S. Williams, Chad Wotkowicz — 1998
• Engineering the mouse genome by site-specific recombination.Daniel METZGER, Robert Feil — 1999
• Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step.Shaorong Chong, G. E. Montello, A. Zhang — 1998

Cited by

• Fyn Signaling Is Compartmentalized to Dopamine D1 Receptor Expressing Neurons in the Dorsal Medial StriatumKendell H. Easley — 2017
• Recombinational Cloning Using Gateway and In-Fusion Cloning SchemesJae-Hong Park, Andrea Throop, Joshua LaBaer — 2015
• Characterization and application of vortex flow adsorption for simplification of biochemical product downstream processingJunfen Ma — 2003
• Identification of two topologically distinct Mu transpososomes: contribution of cis and trans elements to DNA topologyZhiqi Yin — 2006
• Efficient CPP-mediated Cre protein delivery to developing and adult CNS tissuesYorick Gitton, Lorenzo Tibaldi, Edmond Dupont — 2009
• Intein-mediated expression, purification, and characterization of thymosin α1-thymopentin fusion peptide in Escherichia coli.Juan Li, Lei Zheng, Pingli Li — 2012
• Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: A tool for efficient genetic engineering of mammalian genomesMichael Peitz, Kurt Pfannkuche, Klaus Rajewsky — 2002
• Production of D-hydantoinase via surface display and self-cleavage system.Chia-Chi Lin, Tzu-Tsen Liu, Shu‐Chen Kan — 2013
• Design and synthesis of catenated rings based on toroidal DNA structures.Zhaoqi Yang, Dawei Li, Tianhu Li — 2011
• Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purificationShuang Zhang, Zhi Hui Wang, Guo Qiang Chen — 2010
• Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivoPaola Capasso, Marisa Aliprandi, Giuseppe Ossolengo — 2009
• Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag.Holly E. Humphries, Myron Christodoulides, John E. Heckels — 2002
• A phagemid system enabling easy estimation of the combinatorial antibody library size.Seung-Ho Hong, Myung‐Shin Lee, Sun Park — 2004
• Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli.Shamik S. Sharma, Shaorong Chong, Sarah W. Harcum — 2006
• The potential role of self-cleaving purification tags in commercial-scale processes.Baley A. Fong, Wanyi Wu, David W. Wood — 2010
• Production of Nα-acetylated thymosin α1 in Escherichia coliYuantao Ren, Xue-qin Yao, Hongmei Dai — 2011
• Interactions of phage Mu enhancer and termini that specify the assembly of a topologically unique interwrapped transpososome.Zhiqi Yin, Asaka Suzuki, Zheng Lou — 2007
• Expression, purification, phosphorylation and characterization of recombinant human statherin.Barbara Manconi, Tiziana Cabras, Alberto Vitali — 2010
• Self-cleaving fusion tags for recombinant protein productionYifeng Li — 2011
• Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli.Ziyong Sun, Junyong Chen, Hongwei Yao — 2005

Related papers

• Protein splicing facilitated by highly similar inteins from two extreme thermophilesJennie E. Williams, Julie N. Reitter, Kenneth V. Mills — 2012
• Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein.Kenneth V. Mills, Belinda M. Lew, Shuqin Jiang — 1998
• An in vitro screening system for protein splicing inhibitors based on green fluorescent protein as an indicator.Jaya Gangopadhyay, Shuqin Jiang, Henry Paulus — 2003
• Characteristics of protein splicing in trans mediated by a semisynthetic split intein.Belinda M. Lew, Kenneth V. Mills, Henry Paulus — 1999
• Study of Protein Splicing and Intein‐Mediated Peptide Bond Cleavage under High‐Cell‐Density ConditionsSuraj Sharma, A. Zhang, Hui Wang — 2003
• The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and ProteinsA. Sesilja Aranko, Hideo Iwaï — 2021
• In vitro splicing of erythropoietin by the Mycobacterium tuberculosis RecA intein without substituting amino acids at the splice junctions.Jaya Gangopadhyay, Shuqin Jiang, Patrick van Berkel — 2003
• Utilizing of protein splicing phenomenon for optimization of obtaining and purification of the recombinant human growth hormonePetro Starokadomskyy, O. V. Okunev, L. V. Dubey — 2006
• Reversible Inhibition of Protein Splicing by Zinc Ion*Kenneth V. Mills, Henry Paulus — 2001
• Purification of recombinant proteins from E. coli by engineered inteins.Ming‐Qun Xu, Thomas C. Evans — 2003