The signal peptide of the rat corticotropin-releasing factor receptor 1 promotes receptor expression but is not essential for establishing a functional receptor.
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Summary
A mutant of the CRF-R1 lacking the signal peptide was functional and displayed wild-type properties with respect to ligand binding and activation of adenylate cyclase, however, immunoblot analysis and confocal laser scanning microscopy revealed that the mutant receptor was expressed at 10-fold lower levels than the wild- type receptor.
- Type
- article
- Published
- 2005-09-01
- Cited by
- 48
- References
- 31
- Access
- Open access
- OpenAlex
- https://openalex.org/W2074005421
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:27320070
Keywords
Receptor, 5-HT5A receptor, G protein-coupled receptor, Biology, Signal peptide
References
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Cited by
- Splicing and Multiple Binding Proteins in the Corticotropin-Releasing Hormone Stress System.
- Exploring the structure, function & regulation of the human glucagon-like peptide-1 receptor
- Overexpression of claudin‐5 but not claudin‐3 induces formation of trans‐interaction–dependent multilamellar bodies
- Identifying Target Genes related to Respiratory Network Dysfunction in a Mouse Model for the Rett Syndrome
- Functional analysis of the signal peptide of the Protease Activated Receptor 1(PAR1)
- Role of the signal peptide in the synthesis and processing of the glucagon‐like peptide‐1 receptor
- Functional role of the extracellular N-terminal domain of neuropeptide Y subfamily receptors in membrane integration and agonist-stimulated internalization.
- The protease‐activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression
- Inhibition of Biosynthesis of Human Endothelin B Receptor by the Cyclodepsipeptide Cotransin*
- Genetically Encoded Chemical Probes In Cells Reveal the Binding Path of Urocortin-I to CRF Class B GPCR
- Use of Kaede Fusions to Visualize Recycling of G Protein‐Coupled Receptors
- The N-terminal region of the human 5-HT₂C receptor has as a cleavable signal peptide.
- The rat adenine receptor: pharmacological characterization and mutagenesis studies to investigate its putative ligand binding site
- Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity
- The Corticotropin-releasing Factor Receptor Type 2a Contains an N-terminal Pseudo Signal Peptide*
- A Role of Myosin Vb and Rab11‐FIP2 in the Aquaporin‐2 Shuttle
- Diuretic hormone 44 receptor in Malpighian tubules of the mosquito Aedes aegypti: evidence for transcriptional regulation paralleling urination
- Generation and functional characterization of fluorescent, N-terminally tagged CB1 receptor chimeras for live-cell imaging.
- Functional significance of cleavable signal peptides of G protein-coupled receptors.
- The putative signal peptide of glucagon-like peptide-1 receptor is not required for receptor synthesis but promotes receptor expression
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