Fluorescent hybridisation combined with flow cytometry and hybridisation of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes.
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Summary
Faecal samples from 23 healthy individuals were analysed with a similar set of probes targeting six phylogenetic groups using rRNA dot-blot hybridisation and whole cell fluorescent in situ hybridisation (FISH) combined with flow cytometry, providing consistent and complementary information on the structure of the faecal microbiota.
- Type
- article
- Published
- 2003-03-01
- Cited by
- 184
- References
- 30
- Access
- Open access
- OpenAlex
- https://openalex.org/W2073801391
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1271232
Keywords
Biology, Flow cytometry, 16S ribosomal RNA, Ribosomal RNA, Bacteroides
References
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- Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment.
- Human colonic biota studied by ribosomal DNA sequence analysis
- Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
- Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes
- Development of 16S rRNA-Based Probes for theCoriobacterium Group and the Atopobium Cluster and Their Application for Enumeration of Coriobacteriaceaein Human Feces from Volunteers of Different Age Groups
- Molecular Analysis of Intestinal Microbiota Composition to Evaluate the Effect of PEG and Lactulose Laxatives in Humans
- Creating and Maintaining the Gastrointestinal Ecosystem: What We Know and Need To Know from Gnotobiology
Cited by
- Flow cytometric sorting of fecal bacteria after in situ hybridization with polynucleotide probes.
- Influence de la mutation NOD2 et d'un traitement antibiotique sur la colonisation et la pathogénicité d'AIEC dans l'exploration de la maladie de Crohn
- Studies on the detection methods of Campylobacter and faecal indicator bacteria in drinking water
- Influence de la présence et de la composition du microbiote intestinal sur le développement et la prévention des allergies alimentaires
- Molecular methods in microbial ecology.
- Impact des mycotoxines sur le microbiote intestinal humain, cas particulier du déoxynivalénol
- Infant Colic Crying and Gastrointestinal Tract – Causes, Consequences and Cure
- The development of probiotics for use in the ostrich farming industry in South Africa
- Microbiote intestinal et développement de l’obésité : une approche par métagénomique et métabolomique du concept de répondeur et non-répondeur
- Dietary effects on human fecal microbiota
- Separation of bacteria of the Clostridium leptum subgroup from the human colonic microbiota by fluorescence-activated cell sorting or group-specific PCR using 16S rRNA gene oligonucleotides.
- Diversity of the human gastrointestinal microbiota
- Diversité du microbiote et de ses fonctions
- Dégradation des fibres pariétales et système xylanolytique de Bacteroides xylanisolvens XB1AT et Roseburia intestinalis XB6B4, espèces bactériennes du microbiote intestinal humain
- Optimisation de la production d’arabinoxylooligosaccharides d’intérêt biologique à partir de sons de céréales : approches méthodologiques
- Diversity of the human gastrointestinal microbiota : novel perspectives from high throughput analyses
- Analysis of canine small intestinal and fecal microbiota : prevention of ampicillin-induced changes with oral b-lactamase
- Comparative study of a new quantitative real‐time PCR targeting the xylulose‐5‐phosphate/fructose‐6‐phosphate phosphoketolase bifidobacterial gene (xfp) in faecal samples with two fluorescence in situ hybridization methods
- Gaining insight into microbial physiology in the large intestine: a special role for stable isotopes.
- Different metabolic features of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria
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