Tannic acid-mediated osmium impregnation after freeze-substitution: a strategy to enhance membrane contrast for electron tomography.

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Summary

A tannic acid-mediated osmium impregnation method, applied after freeze-substitution, increases membrane contrast in cells for transmission electron microscopy and tomography studies, results in membranes with a proper contrast and equal staining pattern through the depth of the tomograms.

Type
article
Published
2009-04-01
Cited by
45
References
14

Keywords

Electron tomography, Tannic acid, Osmium, Membrane, Staining

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