Tannic acid-mediated osmium impregnation after freeze-substitution: a strategy to enhance membrane contrast for electron tomography.
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Summary
A tannic acid-mediated osmium impregnation method, applied after freeze-substitution, increases membrane contrast in cells for transmission electron microscopy and tomography studies, results in membranes with a proper contrast and equal staining pattern through the depth of the tomograms.
- Type
- article
- Published
- 2009-04-01
- Cited by
- 45
- References
- 14
- OpenAlex
- https://openalex.org/W2073154852
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:28580519
Keywords
Electron tomography, Tannic acid, Osmium, Membrane, Staining
References
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- Dual-axis tomography: an approach with alignment methods that preserve resolution.
- Protein expression dynamics during replicative senescence of endothelial cells studied by 2‐D difference in‐gel electrophoresis
- Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence for mordanting effect
- The effect of tannic acid on electron images of capillary endothelial cell membranes.
- Freeze substitution of high‐pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water
- Template matching as a tool for annotation of tomograms of stained biological structures.
- The use of osmium-thiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods to enhance membrane contrast and preservation in cultured cells.
- Structural studies by electron tomography: from cells to molecules.
- The Emergence of Electron Tomography as an Important Tool for Investigating Cellular Ultrastructure
- Aclar discs: a versatile substrate for routine high‐pressure freezing of mammalian cell monolayers
- Cryo-electron tomography of cells: connecting structure and function
- Endosomal compartmentalization in three dimensions: Implications for membrane fusion
- Conductive staining of biological specimens for scanning electron microscopy with special reference to ligand-mediated osmium impregnation.
Cited by
- A Cholesterol‐Dependent Endocytic Mechanism Generates Midbody Tubules During Cytokinesis
- A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System
- Electron microscopy of the mouse central nervous system.
- Bridging microscopes: 3D correlative light and scanning electron microscopy of complex biological structures.
- Étude par microscopie électronique des mécanismes de transport des nanoparticules de silice au travers d'une barrière endothéliale
- Dynamic separation and recovery of osmium using γ-nano-Al2O3 in a packed column adsorption system
- Focused ion beam scanning electron microscopy in biology
- A Novel Approach for Intracellular 3D Immuno‐Labeling for Electron Tomography
- Gridded Aclar: preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB–SEM
- Spiral Coating of the Endothelial Caveolar Membranes as Revealed by Electron Tomography and Template Matching
- VIS2FIX: A High‐Speed Fixation Method for Immuno‐Electron Microscopy
- A 3D Image Filter for Parameter-Free Segmentation of Macromolecular Structures from Electron Tomograms
- Membrane Contact Sites between Apicoplast and ER in Toxoplasma gondii Revealed by Electron Tomography
- The Stack: A New Bacterial Structure Analyzed in the Antarctic Bacterium Pseudomonas deceptionensis M1T by Transmission Electron Microscopy and Tomography
- Cobblestone HUVECs: a human model system for studying primary ciliogenesis.
- On line vapor generation of osmium based on solution cathode glow discharge for the determination by ICP-OES.
- Investigation of selective and effective recovery of noble metal osmium by adsorption onto nano-Al2O3 particles
- The future is cold: cryo‐preparation methods for transmission electron microscopy of cells
- Endothelial cell senescence is associated with disrupted cell-cell junctions and increased monolayer permeability
- Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment
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