Thrombin and ionomycin can raise platelet cytosolic Ca2+ to micromolar levels by discharge of internal Ca2+ stores: studies using fura-2.
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Summary
Results with fura-2-loaded platelets indicate that mobilisation of internal Ca2+ can contribute a substantial proportion of the early peak [Ca2+]i evoked by thrombin directly confirming the deductions from previous work with different loadings of quin2.
- Type
- article
- Published
- 1986-08-29
- Cited by
- 138
- References
- 13
- OpenAlex
- https://openalex.org/W2070370694
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25952961
Keywords
Ionomycin, EGTA, Thrombin, Chemistry, Platelet
References
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- Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths.
- Muscarinic receptor-induced phosphoinositide hydrolysis at resting cytosolic Ca2+ concentration in PC12 cells
- Agonist-induced calcium transients in cultured smooth muscle cells: measurements with fura-2 loaded monolayers.
- Bidirectional control of cytosolic free calcium by thyrotropin-releasing hormone in pituitary cells
- Using quin2 in cell suspensions.
- Inositol trisphosphate, a novel second messenger in cellular signal transduction
- A new generation of Ca2+ indicators with greatly improved fluorescence properties.
- Stimulus-response coupling in human platelets. Changes evoked by platelet-activating factor in cytoplasmic free calcium monitored with the fluorescent calcium indicator quin2.
Cited by
- d-Indobufen inhibits collagen-induced intracellular calcium mobilization and inositol phosphates formation in human platelets.
- Activation of a K+ conductance by bradykinin and by inositol-1,4,5-trisphosphate in rat glioma cells: involvement of intracellular and extracellular Ca2+.
- Deranged Platelet Calcium Homeostasis in Poorly Controlled IDDM Patients
- Inhibition of platelet function by A02131-1, a novel inhibitor of cGMP-specific phosphodiesterase, in vitro and in vivo.
- The Platelet as a Cа2+-Driven Cell: Mechanisms Which May Modulate Cа2+-Driven Responses
- Signal transduction by the platelet Fc receptor.
- Subsecond calcium dynamics in ADP- and thrombin-stimulated platelets: a continuous-flow approach using indo-1.
- The anti-inflammatory effect of 2-(4-hydroxy-3-prop-2-enyl-phenyl)-4-prop-2-enyl-phenol by targeting Lyn kinase in human neutrophils.
- Platelet cytosolic free calcium concentration, total plasma calcium concentration and blood pressure in human twins: a genetic analysis.
- Calcium efflux from platelet vesicles of the dense tubular system. Analysis of the possible contribution of the Ca2+ pump
- 5‐hydroxy‐2‐(4‐hydroxy‐3‐methoxyphenyl)‐3,7‐dimethoxy‐4H‐chromen‐4‐one (MSF‐2) suppresses fMLP‐mediated respiratory burst in human neutrophils by inhibiting phosphatidylinositol 3‐kinase activity
- Ca2+ efflux from platelets. Control by protein kinase C and the filling state of the intracellular Ca2+ stores.
- The possible involvement of protein phosphatase 1 in thrombin‐induced Ca2+ influx of human platelets
- Variability of the thrombin- and ADP-induced Ca2+ response among human platelets measured using fluo-3 and fluorescent videomicroscopy.
- Effects of barbiturates on human platelet aggregation differ depending on their chemical structures.
- The inhibition of cyclic AMP-mediated exocytosis from rat parotid cells by calcium chelation is due to depression of cellular ATP.
- Differential Activation of Cytosolic Phospholipase A2 (cPLA2) by Thrombin and Thrombin Receptor Agonist Peptide in Human Platelets. EVIDENCE FOR ACTIVATION OF cPLA2 INDEPENDENT OF THE MITOGEN-ACTIVATED PROTEIN KINASES ERK1/2 (*)
- Factor VIII-Mediated Global Hemostasis in the Absence of von Willebrand Factor
- Effect of PP1D-1, a synthetic antiplatelet compound, on rabbit platelets.
- Adrenaline potentiates type 2B von Willebrand factor-induced activation of human platelets by enhancing both the formation and action of thromboxanes.
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