Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Keywords

Biology, Fusion protein, Escherichia coli, Affinity chromatography, Glutathione S-transferase

References

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• The DNA binding subunit of NF-κB is identical to factor KBF1 and homologous to the rel oncogene productAnnamaria Cascetta — 1990
• Maltose binding protein (MBP) fusion proteins with low or no affinity to amylose resins can be single-step purified using a novel anti-MBP monoclonal antibody.Jung‐Hyun Park, Eun‐A Choi, Eun-Wie Cho — 1998
• Target cell specificity of a bacteriocin molecule: a C‐terminal signal directs lysostaphin to the cell wall of Staphylococcus aureus.Takashi Baba, Olaf Schneewind — 1996
• Specification of the Drosophila CNS midline cell lineage: direct control of single-minded transcription by dorsal/ventral patterning genes.Yumi Kasai, Stephanie Stahl, Stephen T. Crews — 1998
• Nuclear genes encoding chloroplast hemoglobins in the unicellular green alga Chlamydomonas eugametosManon Couture, Hélène Chamberland, Benoit St‐Pierre — 1994
• Hierarchy of binding sites for Grb2 and Shc on the epidermal growth factor receptorMaría Luz Martín Peña, José María Sánchez López, Santiago Leguey Galán — 1994
• The accumulation of a cold-regulated chloroplastic protein is light-dependentA. Urbano — 2004
• Protein domains involved in both in vivo and in vitro interactions between human T-cell leukemia virus type I tax and CREBKristen M. McCabe, Serena Yi-Ying Lin, Hiroyuki Tanaka — 1995
• Functional analysis of the Salmonella typhimurium invasion genes invl and invJ and identification of a target of the protein secretion apparatus encoded in the inv locusR. Stroppiana — 1995
• High Level Production of Hybrid Poty virus–like Particles Carrying Repetitive Copies of Foreign Antigens in Escherichia coliJoaquín Vaquero Turcios — 1993
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• DNA-binding specificity of the cut repeats from the human cut-like proteinLeah Gilbert — 1995
• Modulation of the trans-suppression activity of hepatitis C virus core protein by phosphorylationAzyumardi Azra — 1995
• Characterization of the Elk-1 ETS DNA-binding Domain (*)Jesús María Serrano Moracho — 1995
• Virus-encoded cyclinЖилякова Эмма Михайловна — 1994
• Developmental characterization of a Drosophila RNA-binding protein homologous to the human systemic lupus erythematosus-associated La/SS-B autoantigenC Bai, Z Li, Peter Tolias — 1994
• Structural and antigenic analysis of the nucleoprotein of bovine ephemeral fever rhabdovirus.Katie R. Keck — 1994
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• A T7 expression vector for producing N- and C-terminal fusion proteins with glutathione S-transferase.John Doherty — 1994

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