Large α-aminonitrilase activity screening of nitrilase superfamily members: Access to conversion and enantiospecificity by LC–MS

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Summary

A high-throughput screening for the identification of nitrilases demonstrating activity towards alpha-aminonitriles showed the best result was obtained with an enzyme from Burkholderia xenovorans catalysing the enantiospecific hydrolysis of 2-aminovaleronitrile into (S)-norvaline with excellent conversion and enantiomeric excess.

Type
article
Published
2014-09-01
Cited by
7
References
42

Keywords

Nitrilase, Enzyme, Stereochemistry, Chemistry, SUPERFAMILY

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