Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)
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Summary
It is concluded that starting DNA quality is an important consideration for RADSeq; however, the approach remains robust until genomic DNA is extensively degraded.
- Type
- article
- Published
- 2015-11-01
- Cited by
- 142
- References
- 99
- Access
- Open access
- OpenAlex
- https://openalex.org/W2057983728
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:22119987
Keywords
Biology, Restriction enzyme, DNA, DNA sequencing, genomic DNA
References
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Cited by
- Sequence Capture versus Restriction Site Associated DNA Sequencing for Shallow Systematics.
- Consistent differential resource use by sympatric lake (Coregonus clupeaformis) and round (Prosopium cylindraceum) whitefish in Lake Huron: a multi-time scale isotopic niche analysis
- Harnessing the power of RADseq for ecological and evolutionary genomics
- Adapterama IV: Sequence Capture of Dual-digest RADseq Libraries with Identifiable Duplicates (RADcap)
- Adapterama I: Universal Stubs and Primers for Thousands of Dual-Indexed Illumina Libraries (iTru & iNext)
- RADcap: sequence capture of dual‐digest RADseq libraries with identifiable duplicates and reduced missing data
- A field ornithologist's guide to genomics: Practical considerations for ecology and conservation
- High‐throughput microsatellite genotyping in ecology: improved accuracy, efficiency, standardization and success with low‐quantity and degraded DNA
- Serial sequencing of isolength RAD tags for cost-efficient genome-wide profiling of genetic and epigenetic variations
- Bee conservation in the age of genomics
- A comparison of DNA extraction methods for high‐throughput DNA analyses
- Large-Scale Genotyping-by-Sequencing Indicates High Levels of Gene Flow in the Deep-Sea Octocoral Swiftia simplex (Nutting 1909) on the West Coast of the United States
- Lost in parameter space: a road map for stacks
- Future directions and priorities for Arctic bryophyte research
- High-quality genetic mapping with ddRADseq in the non-model tree Quercus rubra
- Tumor diversity and evolution revealed through RADseq
- 2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador
- Adapterama III: Quadruple-indexed, triple-enzyme RADseq libraries for about $1USD per Sample (3RAD)
- Resolving taxonomic turbulence and uncovering cryptic diversity in the musk turtles (Sternotherus) using robust demographic modeling.
- Snake scale clips as a source of high quality DNA suitable for RAD sequencing
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