A maturase-encoding group IIA intron of yeast mitochondria self-splices in vitro.
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Summary
This is the first report of a maturase-encoding intron of either group I or group II that self-splices in vitro, and it requires much higher levels of Mg2+ and added salts for ready detection of splicing activity.
- Type
- article
- Published
- 1992-04-11
- Cited by
- 32
- References
- 1
- Access
- Open access
- OpenAlex
- https://openalex.org/W2056412942
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21099932
Keywords
Group II intron, Intron, RNA splicing, Biology, Group I catalytic intron
References
Cited by
- Reactions catalyzed by group II introns in vitro.
- Group II Introns: Structure and Catalytic Versatility of Large Natural Ribozymes
- Tarantulas and social spiders : a tale of sex and silk
- Expression of intron-encoded maturase-like polypeptides in potato chloroplasts
- Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double‐stranded DNA
- Involvement of DEAD-box proteins in group I and group II intron splicing. Biochemical characterization of Mss116p, ATP hydrolysis-dependent and -independent mechanisms, and general RNA chaperone activity.
- Suppressors of cis-acting splicing-deficient mutations that affect the ribozyme core of a group II intron.
- Multiple tertiary interactions involving domain II of group II self-splicing introns.
- Group II Intron Ribozymes and Metal Ions – A Delicate Relationship
- A group II self-splicing intron from the brown alga Pylaiella littoralis is active at unusually low magnesium concentrations and forms populations of molecules with a uniform conformation.
- Probing Adenine Rings and Backbone Linkages Using Base Specific Isotope-Edited Raman Spectroscopy: Application to Group II Intron Ribozyme Domain V
- Efficient integration of an intron RNA into double-stranded DNA by reverse splicing
- Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.
- RNA splicing in lower eukaryotes.
- A DEAD-box protein alone promotes group II intron splicing and reverse splicing by acting as an RNA chaperone.
- Experimental tests of two proofreading mechanisms for 5'-splice site selection.
- Reverse transcriptase activity associated with maturase-encoding group II introns in yeast mitochondria.
- The biology of yeast mitochondrial introns
- Sequence-based prediction of protein-protein interactions by means of codon usage
- Non-cognate template usage and alternative priming by a group II intron-encoded reverse transcriptase.
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