DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
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Summary
A chemically modified phage T7 DNA polymerase has three properties that make it ideal for DNA sequencing by the chain-termination method; the enzyme is highly processive, catalyzing the polymerization of thousands of nucleotides without dissociating, and processive synthesis with dITP in place of dGTP eliminates band compressions.
- Type
- article
- Published
- 1987-07-01
- Cited by
- 1,768
- References
- 1
- Access
- Open access
- OpenAlex
- https://openalex.org/W2047043554
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:35426897
Keywords
DNA polymerase, Polymerase, Exonuclease, Nucleotide, DNA
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