Physicochemical characterization and purification of cationic lipoplexes.
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Summary
Lipoplexes prepared at a threefold or greater excess of either DOTAP or DNA could be resolved into complexes with a defined stoichiometry and the excess component by sedimentation to equilibrium on sucrose gradients and the negatively charged lipoplex showed increased transfection activity compared to the starting mixture.
- Type
- article
- Published
- 1999-07-01
- Cited by
- 216
- References
- 31
- Access
- Open access
- OpenAlex
- https://openalex.org/W2041153298
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:24471515
Keywords
Cationic polymerization, Characterization (materials science), Chemistry, Biophysics, Materials science
References
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- Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations.
- Formation of ordered domains in membrane-bound DNA.
- Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
- Fusion of liposomes containing a novel cationic lipid, N-[2,3-(dioleyloxy)propyl]-N,N,N-trimethylammonium: induction by multivalent anions and asymmetric fusion with acidic phospholipid vesicles.
- Formation of stable cationic lipid/DNA complexes for gene transfer.
- Biophysical characterization of cationic lipid: DNA complexes.
- Construction of plasmids that express E. coli beta-galactosidase in mammalian cells.
- Mode of formation and structural features of DNA-cationic liposome complexes used for transfection.
- New structures in complex formation between DNA and cationic liposomes visualized by freeze—fracture electron microscopy
- Mechanism of oligonucleotide release from cationic liposomes.
- Complexes between cationic liposomes and DNA visualized by cryo-TEM.
- Induction of fusion in aggregated and nonaggregated liposomes bearing cationic detergents.
- The role of helper lipids in cationic liposome-mediated gene transfer.
- Interactions of mammalian cells with lipid dispersions containing novel metabolizable cationic amphiphiles.
- The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer.
- A novel cationic liposome reagent for efficient transfection of mammalian cells.
- In vitro gene delivery by degraded polyamidoamine dendrimers.
- Freeze fracture studies of membrane lipids.
- Interactions of cationic lipid vesicles with negatively charged phospholipid vesicles and biological membranes.
Cited by
- Analysis of cationic-lipid-plasmid-DNA complexes.
- Fluctuating charged membranes
- Thérapie génique à l'aide de nanocapsules lipidiques PEGylées
- Overview of drug delivery and alternative methods to electroporation.
- On the Transport of Lipoplexes Through Cystic Fibrosis Sputum
- Nanodisk: a versatile drug delivery platform
- The assembly of dialkyldimethylammonium bromide cationic lipids as vesicles or monolayers in presence of poly(ethylene glycol)
- DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes.
- Cationic lipid formaulation of short interference RNA (siRNA) for delivery to respiratory epithelial cells
- Innovative Vectors for Non-viral Gene Therapy using Detergent Dialysis Technique
- Effects of dioleoylphosphatidylethanolamine on the activity and structure of 0-alkylphosphatidylcholine-DNA transfection complexes
- NON-VIRAL GENE DELIVERY WITH pH-SENSITIVE GEMINI NANOPARTICLES: SYNTHESIS OF GEMINI SURFACTANT BUILDING BLOCKS, CHARACTERIZATION AND IN VITRO SCREENING OF TRANSFECTION EFFICIENCY AND TOXICITY
- Physico-chemical analysis of cationic liposome–DNA complexes (lipoplexes) with respect to in vitro and in vivo gene delivery efficiency
- Gemini cationic surfactant-based delivery systems for non-invasive cutaneous gene therapy
- Cryoelectron microscopy of liposomes.
- Characterisation of three novel cationic lipids as liposomal complexes with DNA.
- What Role Can Chemistry Play in Cationic Liposome-Based Gene Therapy Research Today?
- Novel epidermal growth factor directed cationic lipoplexes promote in vitro hepatotropic gene targeting.
- siRNA delivery with precise and biocompatible polycations in neuro2A murine neuroblastoma models
- Lipid composition of cationic nanoliposomes implicate on transfection efficiency
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