A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
Explore this paper's citation graph
Summary
The development of an improved version of YFP named Venus, which contains a novel mutation, F46L, which at 37°C greatly accelerates oxidation of the chromophore, the rate-limiting step of maturation and will enable fluorescent labelings that were not possible before.
- Type
- article
- Published
- 2002-01-01
- Cited by
- 2,903
- References
- 15
- OpenAlex
- https://openalex.org/W2039210066
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:195292666
Keywords
Aequorea victoria, Green fluorescent protein, Yellow fluorescent protein, Fluorescence, Förster resonance energy transfer
References
- Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
- Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein.
- Insulin targeting to the regulated secretory pathway after fusion with green fluorescent protein and firefly luciferase.
- Reducing the Environmental Sensitivity of Yellow Fluorescent Protein
- Dynamic and quantitative Ca2+ measurements using improved cameleons.
- Chromophore formation in green fluorescent protein.
- Monitoring secretion in real time: capacitance, amperometry and fluorescence compared.
- Circularly permuted green fluorescent proteins engineered to sense Ca2+
- Simultaneous evanescent wave imaging of insulin vesicle membrane and cargo during a single exocytotic event.
- Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy.
- Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine).
- Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling
- Mechanism and Cellular Applications of a Green Fluorescent Protein-based Halide Sensor*
- Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis.
- GREEN FLUORESCENT PROTEIN
Cited by
- In vivo imaging in humanized mice.
- Nuclear organization and the control of HIV-1 transcription.
- The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
- Use of fluorescence microscopy to analyze genetic circuit dynamics.
- Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae
- Transgenic frogs expressing the highly fluorescent protein venus under the control of a strong mammalian promoter suitable for monitoring living cells
- Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
- The Family of GFP-Like Proteins: Structure, Function, Photophysics and Biosensor Applications. Introduction and Perspective
- Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels.
- Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain.
- SYD-2 Liprin-α organizes presynaptic active zone formation through ELKS
- Two Oppositely Localised Frizzled RNAs as Axis Determinants in a Cnidarian Embryo
- Quantitative detection of protein expression in single cells using droplet microfluidics.
- Rab6 regulates transport and targeting of exocytotic carriers.
- Recent advances using green and red fluorescent protein variants
- Bimolecular complementation reveals that glycoproteins gB and gH/gL of herpes simplex virus interact with each other during cell fusion
- Expression of the nuclear oncogene Ski in patients with acute myeloid leukemia treated with all-trans retinoic acid
- Modulating Vesicle Priming Reveals that Vesicle Immobilization Is Necessary but not Sufficient for Fusion-Competence
- FLIM and emission spectral analysis of caspase-3 activation inside single living cell during anticancer drug-induced cell death
- Probing Alzheimer amyloid peptide aggregation using a cell-free fluorescent protein refolding method.
Related papers
- GREEN FLUORESCENT PROTEIN
- In planta comparative analysis of improved green fluorescent proteins with reference to fluorescence intensity and bimolecular fluorescence complementation ability
- Fluorescence resonance energy transfer using color variants of green fluorescent protein.
- Engineering FRET constructs using CFP and YFP.
- Visualization of ternary complexes in living cells by using a BiFC-based FRET assay
- Engineering color variants of green fluorescent protein (GFP) for thermostability, pH-sensitivity, and improved folding kinetics
- Use of green fluorescent protein (GFP) to study cellular dynamics. Constructing GFP-tagged motor enzymes.
- Imaging FRET between spectrally similar GFP molecules in single cells
- Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells.
- Improving FRET dynamic range with bright green and red fluorescent proteins