The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.
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Summary
The reconstitution of a pathway that leads to the apoptotic changes in nuclei by using recombinant DNA fragmentation factor (DFF), a heterodimeric protein of 40 and 45 kDa, suggests that DFF40 is sufficient to trigger both DNA fragmentation and chromatin condensation during apoptosis.
- Type
- article
- Published
- 1998-07-21
- Cited by
- 558
- References
- 31
- Access
- Open access
- OpenAlex
- https://openalex.org/W2038055601
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:31779345
Keywords
DNA fragmentation, Apoptotic DNA fragmentation, Chromatin, Fragmentation (computing), Prophase
References
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- CPP32/Apopain Is a Key Interleukin 1 Converting Enzyme-like Protease Involved in Fas-mediated Apoptosis (*)
- Yama/CPP32β, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase
- Activation of Multiple Interleukin-1β Converting Enzyme Homologues in Cytosol and Nuclei of HL-60 Cells during Etoposide-induced Apoptosis*
- CED-4--the third horseman of apoptosis.
- Chromatin fractionation procedure that yields nucleosomes containing near-stoichiometric amounts of high mobility group nonhistone chromosomal proteins.
- Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells
- Caspases: killer proteases.
- Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
- The involvement of histone H1[0] in chromatin structure.
Cited by
- Caspase-independent commitment phase to apoptosis in activated blood T lymphocytes: reversibility at low apoptotic insult.
- Programmed Cell Death in C. elegans
- Role of factors downstream of caspases in nuclear disassembly during apoptotic execution.
- The Molecular Mechanism of Programmed Cell Death in C. elegans
- A new method for protein coexpression in Escherichia coli using two incompatible plasmids.
- Phosphorylation of histones triggers DNA fragmentation in thymocyte undergoing apoptosis induced by protein phosphatase inhibitors.
- Modeling Apoptotic Chromatin Condensation in Normal Cell Nuclei
- Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities
- Differential Efficacy of Caspase Inhibitors on Apoptosis Markers during Sepsis in Rats and Implication for Fractional Inhibition Requirements for Therapeutics
- Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G
- Cell death effects of resin-based dental material compounds and mercurials in human gingival fibroblasts
- Endonucleases and apoptosis in animals
- Alternative pre-mRNA splicing and regulation of programmed cell death.
- Ionic and cofactor requirements for the activity of the apoptotic endonuclease DFF40/CAD
- Pathways of myocyte death: implications for development of clinical laboratory biomarkers.
- The co-modulation of apoptosis and the cell cycle
- Structure and evolution of ubiquitin and ubiquitin-related domains.
- Caspase-3-dependent and caspase-3-independent pathways leading to chromatin DNA fragmentation in HL-60 cells
- Flow cytometric detection of activated caspase-3.
- Defective apoptotic signal transduction pathway downstream of caspase-3 in human B-lymphoma cells: A novel mechanism of nuclear apoptosis resistance.
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