A TALE nuclease architecture for efficient genome editing
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Summary
This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.
- Type
- article
- Published
- 2011-02-01
- Cited by
- 2,121
- References
- 52
- OpenAlex
- https://openalex.org/W2025074364
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:53549397
Keywords
Genome editing, Transcription activator-like effector nuclease, Biology, Nuclease, Effector
References
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- Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery
- Enhanced protein production by engineered zinc finger proteins
- Genome editing with engineered zinc finger nucleases
- A Bacterial Effector Acts as a Plant Transcription Factor and Induces a Cell Size Regulator
- Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells
- Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures
- Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology
- Highly efficient endogenous human gene correction using designed zinc-finger nucleases
- How Xanthomonas type III effectors manipulate the host plant.
- Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors
- Highly efficient gene targeting of expressed and silent genes in human ESCs and iPSCs using zinc finger nucleases
Cited by
- The Sleeping Beauty transposon system for clinical applications
- TALENs: a widely applicable technology for targeted genome editing
- A novel promoterless gene targeting vector to efficiently disrupt PRNP gene in cattle.
- TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA targeting proteins
- Modeling human disease with pluripotent stem cells: from genome association to function.
- The Presenilin-1 ΔE9 mutation results in reduced γ-secretase activity, but not total loss of PS1 function, in isogenic human stem cells
- DNA Replicons for Plant Genome Engineering[W][OPEN]
- Engineered TAL Effector modulators for the large-scale gain-of-function screening
- Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide–RNAs
- Functional implications of a psychiatric risk variant within CACNA1C in induced human neurons
- Molecular scissors and their application in genetically modified farm animals
- TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice.
- DNA sequence-specific binding of CENP-B enhances the fidelity of human centromere function
- Nanomedicine-mediated cancer stem cell therapy.
- The Development of TALE Nucleases for Biotechnology
- Gene editing technology as an approach to the treatment of liver diseases
- Reactivation of Latent HIV-1 Expression by Engineered TALE Transcription Factors
- Quantification of dopaminergic neuron differentiation and neurotoxicity via a genetic reporter
- TALE proteins search DNA using a rotationally decoupled mechanism.
- Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo
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