Purification and Characterization of Hypoxia-inducible Factor 1 (*)
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Summary
The biochemical purification of HIF-1 from Epo-producing Hep3B cells and non-Epo-producing HeLa S3 cells concludes that in both cobalt chloride-treated HeLa cells and hypoxic Hep3 B cells HIF -1 is composed of two different subunits: 120-kDa Hif-1α and 91-94-k da HIF,1β.
- Type
- article
- Published
- 1995-01-20
- Cited by
- 2,096
- References
- 35
- Access
- Open access
- OpenAlex
- https://openalex.org/W2024206845
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:41659164
Keywords
Erythropoietin, Molecular biology, Enhancer, Biology, DNA
References
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- Hypoxia increases rate of transcription and stability of tyrosine hydroxylase mRNA in pheochromocytoma (PC12) cells.
- Desferrioxamine induces erythropoietin gene expression and hypoxia-inducible factor 1 DNA-binding activity: implications for models of hypoxia signal transduction.
- Purification of nuclear factor I by DNA recognition site affinity chromatography.
- Characterization of hypoxia-inducible factor 1 and regulation of DNA binding activity by hypoxia.
- Regulation of inducible and tissue-specific gene expression.
- Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene.
- Affinity purification of sequence-specific DNA binding proteins.
- Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography.
- Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
- Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein.
- Coordinate reciprocal trends in glycolytic and mitochondrial transcript accumulations during the in vitro differentiation of human myoblasts
- Hypoxia induces endothelin gene expression and secretion in cultured human endothelium.
- Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis
- A 24-base-pair sequence 3' to the human erythropoietin gene contains a hypoxia-responsive transcriptional enhancer.
- A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels.
- Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism.
Cited by
- The role of lactate dehydrogenase B in aerobic glycolysis-mediated resistance to AB toxicity
- Expression of Vascular Endothelial Growth Factor-A and its Receptors in Uteroplacental Tissues of the Normotensive and Hypertensive Pregnant Rat
- Ah receptor signaling pathways.
- A new biological strategy for high productivity of recombinant proteins in animal cells by the use of hypoxia-response enhancer.
- Hypoxia Regulation of Gene Transcription
- Role of nitric oxide in the regulation of HIF-1α expression during hypoxia
- Angiogenesis of the heart
- Podocyte expression of hypoxia-inducible factor (HIF)-1 and HIF-2 during glomerular development.
- Correlation between VEGF and HIF-1alpha expression in human oral squamous cell carcinoma.
- Hypoxia and hypoxia inducible factors (HIF) as important regulators of tumor physiology.
- Hypoxia-inducible factor 1-related diseases and prospective therapeutic tools.
- Constitutively active HIF-1alpha improves perfusion and arterial remodeling in an endovascular model of limb ischemia.
- Increased blood vessel density provides the mole rat physiological tolerance to its hypoxic subterranean habitat
- Hypoxia inducible factor-1: regulation by nitric oxide in posthypoxic microvascular endothelium.
- Differential Regulation of the Transcriptional Activities of Hypoxia-Inducible Factor 1 Alpha (HIF-1α) and HIF-2α in Stem Cells
- Regulation of angiogenesis by hypoxia-inducible factor 1.
- Novel Conditioning Protocols Focusing on Oxygen Manipulation to Enhance Stem Cell Transplantation
- Highly Efficient Transfection of Rat Cortical Neurons Using Carbosilane Dendrimers Unveils a Neuroprotective Role for HIF-1α in Early Chemical Hypoxia-Mediated Neurotoxicity
- Distinctions and similarities of cell bioenergetics and the role of mitochondria in hypoxia, cancer, and embryonic development.
- HIFs as central regulators of gastric cancer pathogenesis
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