Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction.
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Summary
This work has devised a simple method, not dependent on primer ratios, for generating single-stranded DNA from double-stranding PCR products and shows a portion of the DNA sequence of the gene encoding GAPDH from Salmonella typhimurium.
- Type
- article
- Published
- 1989-07-25
- Cited by
- 306
- References
- 0
- Access
- Open access
- OpenAlex
- https://openalex.org/W2018340379
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9712037
Keywords
Exonuclease, Biology, Polymerase chain reaction, DNA polymerase, Polymerase
References
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