Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
Explore this paper's citation graph
Summary
The theoretical basis and some practical guidelines for simple, rigorous analysis of FPR experiments are presented and some model experiments on aqueous solutions of rhodamine 6G are described.
- Type
- article
- Published
- 1976-09-01
- Cited by
- 2,531
- References
- 17
- Access
- Open access
- OpenAlex
- https://openalex.org/W2017934591
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:23926882
Keywords
Fluorophore, Photobleaching, Fluorescence recovery after photobleaching, Chemistry, Fluorescence
References
- Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts.
- Dynamics of fluorescence marker concentration as a probe of mobility.
- Some Applications in Physics of the P Function
- Lateral diffusion of rhodopsin in the photoreceptor membrane
- Measurement of membrane protein lateral diffusion in single cells.
- A microfluorimetric study of translational diffusion in erythrocyte membranes.
- Fluorescence correlation spectroscopy. II. An experimental realization
- Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching.
- Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A.
- Rotational and translational diffusion in membranes.
- Statistical accuracy in fluorescence correlation spectroscopy
- Lateral diffusion of concanavalin A receptors in the plasma membrane of mouse fibroblasts.
- Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations.
- Fluorescence correlation spectroscopy. I. Conceptual basis and theory
- Mammalian plasma membranes
- Conduction of Heat in Solids
Cited by
- The lipid environment of the nicotinic acetylcholine receptor in native and reconstituted membranes.
- Measurements of diffusion and clustering of integrin receptors in the plasma membrane of live cells using fluorescence-based techniques
- Antigen-specific mouse lymphocyte stimulation by DNP-conjugated T-independent antigens studied by photobleaching recovery.
- Fluorescently labelled molecules as probes of the structure and function of living cells
- Single Molecule Probe Scanning Optical Force Imaging Microscopefor Viewing Live Cells
- Analysis of simulated and experimental fluorescence recovery after photobleaching. Data for two diffusing components.
- Microtubule inhibitors differentially affect translational movement, cell surface expression, and endocytosis of transferrin receptors in K562 cells
- Action of DNA repair endonuclease ERCC1/XPF in living cells.
- Phosphatidylserine/Cholesterol Bilayers Supported on a Polycation/Alkylthiol Layer Pair.
- Accessing Molecular Dynamics in Cells by Fluorescence Correlation Spectroscopy
- Diffusion of macromolecules and virus-like particles in human cervical mucus.
- Marching at the front and dragging behind
- Emerging issues of connexin channels: biophysics fills the gap
- Micropattern formation in supported lipid membranes.
- Cell Adhesion Biophysics on Dynamic Polymer Constructs
- Overview of confocal microscopy.
- Quick tour of fluorescence correlation spectroscopy from its inception.
- Derivation of a closed form analytical expression for fluorescence recovery after photo bleaching in the case of continuous bleaching during read out
- Dynamic regulation of ERK2 nuclear translocation and mobility in living cells
- Hsp104-Dependent Remodeling of Prion Complexes Mediates Protein-Only Inheritance
Related papers
- Measuring Dynamics of Nuclear Proteins by Photobleaching
- Revealing protein dynamics by photobleaching techniques.
- Photobleaching approaches to investigate diffusional mobility and trafficking of Ras in living cells.
- Photobleaching of Rhodamine 6G in polyacrylonitrile matrix
- Study of the influence of triplet quencher on the photobleaching of rhodamine-6G
- Evidence for lack of damage during photobleaching measurements of the lateral mobility of cell surface components.
- Measuring Protein Mobility by Photobleaching GFP Chimeras in Living Cells
- Photobleaching GFP reveals protein dynamics inside live cells.
- Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy