Graphite powder as an alternative or supplement to reversed‐phase material for desalting and concentration of peptide mixtures prior to matrix‐assisted laser desorption/ionization‐mass spectrometry
Explore this paper's citation graph
Summary
The use of graphite powder packed in constricted GELoader tips as an alternative to RP microcolumns for desalting and concentration of peptide mixtures prior to MS is reported here and it is shown that samples contaminated with small biological polymers can readily be analyzed using graphite Powder rather than RP microColumns.
- Type
- article
- Published
- 2002-09-01
- Cited by
- 115
- References
- 0
- OpenAlex
- https://openalex.org/W2016031823
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:26913093
Keywords
Chromatography, Mass spectrometry, Chemistry, Matrix-assisted laser desorption/ionization, Graphite
References
No references recorded for this paper.
Cited by
- Sample preparation by in-gel digestion for mass spectrometry-based proteomics
- Identification and characterization of N-glycosylated proteins using proteomics.
- Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences
- Synergistic effect of graphene oxide/MWCNT films in laser desorption/ionization mass spectrometry of small molecules and tissue imaging.
- Chemically modified diamond-like carbon (DLC) for protein enrichment and profiling by MALDI-MS
- Comparative proteome analysis of a human liver cell line stably transfected with hepatitis D virus full-length cDNA.
- New insights into mutable collagenous tissues: an inspiring model for tissue regeneration
- Proteomic Analysis of the Response of Pseudomonas Aeruginosa PAO1 to the Cell to Cell Signaling Molecule Trans, Trans-farnesol of Candida Albicans
- Energy transduction by respiratory complex I
- The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
- Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)*
- Equal ratio of graphite carbon to activated charcoal for enrichment of N-glycopeptides prior to matrix-assisted laser desorption/ionization time-of-flight mass spectrometric identification.
- A convenient purification and preconcentration of peptides with alpha-cyano-4-hydroxycinnamic acid matrix crystals in a pipette tip for matrix-assisted laser desorption/ionization mass spectrometry.
- Metodologías fosfoproteómicas útiles en estudios clínicos
- Proteome of Gluconacetobacter diazotrophicus co-cultivated with sugarcane plantlets.
- Global methods for protein glycosylation analysis by mass spectrometry.
- High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
- The impact of chromatography and mass spectrometry on the analysis of protein phosphorylation sites
- Protein chemical characterization of Gc globulin (vitamin D-binding protein) isoforms; Gc-1f, Gc-1s and Gc-2.
- Serum protein profiling by miniaturized solid-phase extraction and matrix-assisted laser desorption/ionization mass spectrometry.
Related papers
- Comparison of dissolution procedure for the determination of heavy metals in polymers
- Sample preparation procedures for accurate and precise isotope analysis of Pb in peat by multiple collector (MC)-ICP-MS
- A rapid microtechnique for the preparation of biological material for the simultaneous analysis of calcium, magnesium and protein.
- Sample Preparation for Detection of Different Bacterial Strains by Matrix‐Assisted Laser Desorption/Ionization Time‐of‐Flight (MALDI‐TOF) Mass Spectrometry
- Independent assessment of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) sample preparation quality: Effect of sample preparation on MALDI-MS of synthetic polymers.
- Sample preparation strategies for low-volume determinations inIn vitro studies of proteins by use of MALDI-TOF MS
- Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target.
- Sample preparation and culture condition effects on MALDI-TOF MS identification of bacteria: A review.
- MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) of skin: Aspects of sample preparation.
- A MALDI sample preparation method suitable for insoluble polymers