Direct gene transfer into mouse muscle in vivo.
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Summary
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo and expression was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions.
- Type
- article
- Published
- 1990-03-23
- Cited by
- 4,134
- References
- 14
- OpenAlex
- https://openalex.org/W2014495927
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:11288078
Keywords
Luciferase, Chloramphenicol acetyltransferase, Molecular biology, In vivo, Gene expression
References
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- Direct transfection of viral and plasmid DNA into the liver or spleen of mice.
- Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer.
- Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
- Transfer of a protein encoded by a single nucleus to nearby nuclei in multinucleated myotubes.
- Receptor-mediated gene delivery and expression in vivo.
- Increased expression of DNA cointroduced with nuclear protein in adult rat liver.
- Penetration of horseradish peroxidase into the terminal cisternae of frog skeletal muscle fibers and blockade of caffeine contracture by Ca++ depletion
Cited by
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- Plasmid vaccines and therapeutics: from design to applications.
- Cytokine gene expression in epidermis with biological effects following injection of naked DNA
- Bacterial Lipopolysaccharide Copurifies with Plasmid DNA: Implications for Animal Models and Human Gene Therapy
- Cardiac gene transfer by intracoronary infusion of adenovirus vector-mediated reporter gene in the transplanted mouse heart.
- Serum and colostrum antibody responses induced by jet-injection of sheep with DNA encoding a Cryptosporidium parvum antigen.
- Genetic Immunization
- DNA immunization confers protective immunity on mice challenged intravaginally with herpes simplex virus type 2.
- Transgene expression of plasmid DNAs directed by viral or neural promoters in the rat brain.
- DNA vaccination using expression vectors carrying FIV structural genes induces immune response against feline immunodeficiency virus.
- Neonatal DNA immunization with a plasmid encoding an internal viral protein is effective in the presence of maternal antibodies and protects against subsequent viral challenge
- Intradermal immunization with a bovine herpesvirus-1 DNA vaccine induces protective immunity in cattle.
- Lipofection of cultured mouse muscle cells: a direct comparison of Lipofectamine and DOSPER
- Source and route of exposure influence infectivity of a molecular clone of human T cell leukemia virus type I.
- Studying Amphibian Metamorphosis by in Vivo Gene Transfer a
- Intranasal administration of human immunodeficiency virus type‐1 (HIV‐1) DNA vaccine with interleukin‐2 expression plasmid enhances cell‐mediated immunity against HIV‐1
- Plasmid DNA Malaria Vaccine: The Potential for Genomic Integration after Intramuscular Injection
- Marked Enhancement of Direct Respiratory Tissue Transfection by Aurintricarboxylic Acid
- Naked plasmid‐mediated gene transfer to skeletal muscle ameliorates diabetes mellitus
- Coimmunization with IFN- gamma or IL-2, but Not IL-13 or IL-4 cDNA Can Enhance Th1-Type DNA Vaccine-Induced
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