Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant.
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Summary
Two cassettes with tetracycline-resistance (TcR) and kanamycin-res resistance (KmR) determinants have been developed for the construction of insertion and deletion mutants of cloned genes in Escherichia coli.
- Type
- article
- Published
- 1995-01-01
- Cited by
- 2,025
- References
- 20
- OpenAlex
- https://openalex.org/W2014361215
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:22463989
Keywords
Biology, Genetics, FLP-FRT recombination, Recombinase, Gene
References
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- Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression
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- Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.
- An "in-out" strategy using gene targeting and FLP recombinase for the functional dissection of complex DNA regulatory elements: analysis of the beta-globin locus control region.
- Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.
- In vivo excision and amplification of large segments of the Escherichia coli genome.
- Control of cloned gene expression by promoter inversion in vivo: construction of the heat-pulse-activated att-nutL-p-att-N module.
- The transposon Tn5 carries a bleomycin-resistance determinant.
- Gene transfer with subsequent removal of the selection gene from the host genome.
- Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes
- The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila.
- Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors.
- Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
- Mutants of Escherichia coli Lacking Endonuclease I, Ribonuclease I, or Ribonuclease II
- Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
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- Deletion of Open Reading Frame UL26 from the Human Cytomegalovirus Genome Results in Reduced Viral Growth, Which Involves Impaired Stability of Viral Particles
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- Members of the Conserved DedA Family Are Likely Membrane Transporters and Are Required for Drug Resistance in Escherichia coli
- Phenotypic Characterization of Salmonella RyhB-1 Mutations that Modulate Target Regulation
- Constraint‐based modeling of heterologous pathways: Application and experimental demonstration for overproduction of fatty acids in Escherichia coli
- NlpI-mediated modulation of outer membrane vesicle production through peptidoglycan dynamics in Escherichia coli
- PhoB activation in non-limiting phosphate condition by the maintenance of high polyphosphate levels in the stationary phase inhibits biofilm formation in Escherichia coli.
- Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources
- Escherichia coli cytochrome c peroxidase is a respiratory oxidase that enables the use of hydrogen peroxide as a terminal electron acceptor
- Identification of novel genes involved in high hydrostatic pressure resistance of Escherichia coli.
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